344 Antisera for Birds' Eggs 



Seven tests were carried out with dilutions of Reptile egg-albumins 

 of which 28 / were positive. 



No positive reactions were obtained with 10, and 25 tests, respect- 

 ively with Amphibian, and Fish egg-albumins. 



The 215 tests with Reptilian sera yielded noteworthy results. All 

 the antisera tested reacted to some extent with Chelonian bloods, but 

 only anti-fowl's egg serum (I) gave any positive results with the other 

 Reptilia. 



Reptilian Sera Tests 1'ositive reactions ( Jood reactions 



Chelonia 55 23-5 "/ ''/ 



Crocodilia 15 13 ,, 7 ,, 



Lacertilia 47 2 



Ophidia 98 2 1 ,, 



Out of 64 experiments with Amphibian sera only one showed am 

 trace of reaction, namely, a very feeble clouding when anti-1'owl's-egg 

 serum was added to the diluted blood of the Toad. 



130 tests with Fish, and 46 with Crustacean sera gave no indications 

 of positive reactions. 



These experiments, therefore, indicate a relationship between the 

 Aves and Chelonia, and a less marked one between the Aves and 

 Crocodilia. As indicated by this test the relationship between the 

 Aves and Lacertilia and Ophidia is very remote (see pp. 200 207, 

 216, 300305). 



C. Quantitative Measurements with Antisera to 

 Birds' Egg-albumin. 



These experiments were conducted by the method which has already 

 been described (p. 315), and the results of the measurements of precipita 

 in each case are stated in c.c. in the second column. The percentages 

 given in the last column are calculated on the basis of the amount 

 of precipitum obtained, the amount given by an antiseruni with its 

 homologous blood being taken as 100%- In most cases this figure 

 has not been exceeded when the antiseruni acted on a non-homologous 

 blood. 



