Graham-Sin it It 347 



and Silver Pheasant egg-albumins, marked reactions with Greenfinch, 

 Moorhen, and Hedge-sparrow, and small with Emu. 



The quantitative method fails to bring out the delicate reactions 

 which can be determined by the qualitative method, but in the case of 

 the major reactions differences can be recognised which are not apparent 

 by the latter method. By its means closely allied sera and egg-albumins 

 can be differentiated, which cannot be done with certainty by the quali- 

 tative method unless low dilutions are made use of. The results of the 

 few quantitative experiments which have been made are exactly in 

 accord with those obtained by the qualitative method. 



II. Antisera for Reptilia. 



Qualitative Tests with Anti-Reptilian Sera. 



A. Anti-Chelonian Sera. 

 (1) Tests with Anti-Tortoise Sennit. 



This antiserum was prepared by intraperitoneal injections of the 

 serum of the Tortoise (Testudo ibera) into a Rabbit. The serum used 

 was obtained from healthy tortoises. 



Percentage 

 of positive 

 Material tested *? 



'26 F.gy-Albituiittx 



(a) 15 Birds' egg-albumius 8 2 (IS 11 ,;) 6(33 (I J 

 (6) 2 Reptile 1 1 



(c) 3 Amphibian 21 



(d) 6 Fish ,,(! 

 121) Hluod .S'era 



(ISChelonia 2 . 5(33/ ) 2(13"/ ) 6(40/o) 87 



. . 4 Crocodilia 3 1 25 



OOEc P tlh Ml3Lacertilia 13 . 



1 28 Ophidia 2(1 1 1 



18 Amphibia 17 1 



3<J 1'isces 3'J 



12 Arthropod* 12 



This was the most powerful anti-chelonian serum made. Large 

 reactions occurred with 87 % of the Chelonian bloods tested, but slight 

 reactions only with a few other specimens of Reptilian blood. 4<i" of 

 the Birds' egg-albumins tested gave some indications of clouding. 



