348 Antisera for Reptilia 



(2) Tests with Anti-Tortitise tienuii. 



This antiserum was prepared by intravenous injections of the serum 

 of a giant Tortoise (Testudo vicina) into a Rabbit. The serum used was 

 collected after the death of the tortoise from natural causes. 



Percentage 



of positive 



Material tested * + + reactions 



24 Egg-Albumins 



(a) 13 Birds' egg-albumins 10 . 2(14"/ ) 1 (7/,,) 23 



(6) 2 Reptile 2 



(c) 3 Amphibian,, 3 ... . o 



(d) 6 Fish 6 

 128 Blood Sera 



(16 Chelonia 5 5 (33 "/) 2(13",,) . 3 (20/ ) 66 



4 Crocodilia 211 . . 50 

 ,- T .... 



12 Lacertiha 11 1 8 



280phidia 24 2 2 12 



18 Amphibia 18 ... Q 



39 Pisces 39 



12 Arthropoda 12 



Only a small quantity of the tortoise serum was available for pre- 

 paring this antiseruin, which was considerably, weaker than the preceding 

 one. The results of the tests are, however, very much alike. In this 

 case large reactions with Chelonian sera were obtained in 66 / as 

 against 87 % i n tno former, and in 23 / as against 46 / of Birds' egg- 

 albumins. Again, the few reactions obtained amongst the rest of the 

 Reptilia were feeble, and the results of tests with Reptile egg-albumins 

 were negative. 



(3) Tests with Anti-Turtle Serum. 



This antiserum was prepared by the intraperitoneal injection of the 

 blood of a Turtle (Chelone midas) into a Rabbit. The turtle was healthy. 



Although this was a very powerful antiserum, giving well-marked 

 reactions with 66 / of the Chelonia, yet only 20 / of the Birds' egg- 

 albumins gave any indication of clouding, and in each case the reaction 

 was very feeble. Fewer positive results were obtained with the other 

 specimens of Reptilian bloods than with cither of the anti-tortoise srni. 



Omitting very feeble cloudings the anti-chelonian sera give well- 

 marked reactions with the Chelonia and moderate reactions with the 

 Crocodilia. With Birds' egg-albumins moderate cloudings are obtained, 



