Graham-Sin ft/t 



This antiserum was a powerful one, but was very slightly opalescent. 

 As shown in the above table, however, it was entirely confined to the 

 Reptilia in its reactions. The maximum reactions occurred amongst 

 the Lacertilia, though fairly well-marked clouding and precipita were 

 obtained with the Ophidia as well as the Chelonia and Crocodilia. It 

 is unfortunate that both the Lacertilian antisera were somewhat un- 

 satisfactory, the first being very weak, and the second slightly opalescent, 

 rendering the determination of slight reactions difficult. In the latter 

 case all very slight cloudings have been disregarded, and a clouding 

 usually described by the sign * has here been marked as ?. The 

 larger reactions were however well marked and unmistakeable. 



D. Anti-Ophidian Sera. 



a. Qualitative Tests. 

 (1) Tests ivith Anti-Snake Serum. 



This antiserum was obtained by the intraperitoneal injection into a 

 Rabbit of Snake serum (Tropidonotus nutri.r). The serum had been 

 previously heated to 55 C. in order to destroy its toxic properties. 



Percentage 



Material tested + + "tjSSS? 

 22 Eiig-Albnmin 



(H) 10 Birds' egg-albumins 9.1... 10 



(b) 2 Reptile 1 1 100 



(<) 3 Amphibian 3 . . . ' . 



(it) 7 Fish 7 ..... 

 127 lilooil Sera 



115 Chelonia 13 1 1 .13 



4 Crocodilia 2 1 1 ... 60 

 in T 



12 Lacertilia 7 5 40 



28 Ophidia 15 7 4 1 1 40 



17 Amphibia 17 



39 Pisces 39 ..... 



12 Arthropoda 12 .. 



This was a moderately powerful antiserum, producing its maximum 

 effects amongst the Ophidia. The few reactions obtained with Chelonian, 

 Crocodilian and Lacertilian bloods were all feeble. One bird's egg- 

 albumin (Moorhen) gave a small reaction, but a well-marked clouding 

 followed by a deposit was obtained with a dilution of snake's egg- 

 albumin, and a smaller clouding with tortoise egg-albumin. 



