356 Antisera for Amphibia, Pisces 



These quantitative Tests, as in the case of those made with antisera 

 to Avian egg-albumins, are almost entirely in accord with the quali- 



tative experiments. 



III. Anti-Amphibian Serum. 



Tests with Anti-Frog Serum. 



This antiserum was prepared by Dr Nuttall by the intraperitoneal 

 injection of Frog's blood (Rana temporaria) into Rabbits. 



Percentage 

 of positive 

 Materials tested * ? x + + reactions 



18 Egg-Albumim (1 Amphibian) 18 ... o 

 70 Blood Sera 



31 Eeptilia 31 ... 



I 4 Urodela 4 ... o 



' Amphlb ' a 111 Anura 5 4 (36%) . 2 (18/ ) 54 



15 Pisces 15 ... 



9 Crustacea 9 ... o 



This was a moderately powerful antiserum. Good reactions occurred 

 only with two specimens of the blood of the Frog (Rana temporaria) 

 and moderate with four examples of Frog's blood (1 Rana temporaria 

 and 3 R. tigrina). All other tests were negative (see p. 210). 



IV. Anti-Fish Serum. 



Tests with Anti-Ammocoetes Serum. 



This antiserum was prepared by the intraperitoneal injection into a 

 Rabbit of Ammocoetes serum, which had been dried on filter-paper, and 

 dissolved out by means of salt solution. 



Percentage 

 of positive 

 Material tested ? * x + + reactions 



23 Egg-Albumins 23 ' .... 



131 Blood Sera 



60 Beptilia 60 ..... 



17 Amphibia 17 ..... 



(12 Chondropterygii 11 1 .... 8 



26 Teleostei 24 2 .... 8 



1 Cyclostomata 1 100 



15 Arthropoda 15 ..... o 



