Graham-Smith 357 



This antiserum was only of moderate power, producing with its 

 homologous serum a medium clouding, followed by a well-marked pre- 

 cipitum. Only three doubtful cloudings were, however, obtained with 

 other fish sera, and other tests made with it were entirely negative. 

 Several tests were made with Limulus, and Arthropod sera, but with 

 negative results. 



V. Anti-Ascidian Serum. 



Tests with Anti-Ascidian Serum. 



This antiserum was obtained by the intraperitoneal injection into a 

 Guinea-pig of Ascidian extract. The extract was made by cutting large 

 fixed Ascidians into small pieces, and extracting these with salt solution. 

 The supernatant fluid which was used in these experiments was clear 

 and of a light yellow tint. 



Percentage 

 of positive 

 *? * x + + reactions 





 

 

 1.1. 100 



This antiserum produced a well-marked precipitum with its homo- 

 logous extract, and a slight reaction with an extract of a species of small 

 fixed Ascidian, but failed to give any trace of reaction with any of the 

 blood sera or egg-albumins. 



VI. Anti-Crustacean Sera. 



(1) Tests with Anti-Lobster Serum. 



This antiserum was made by Dr Nuttall by the injection of Lobster 

 (Homarus vulgaris) serum into Rabbits. Percentage 



of positive 

 Material tested . ? * reactions 



14 Egg-Albumins 



(a) 13 Birds' egg-albumins 13 

 (fc) 1 Reptile 1 



53 Blood Sera 



20 Keptilia 18 



12 Amphibia 10 2 



12 Pisces 12 





9 Crustacea 



8 . I 5 (M'/J 1 77 



