358 



Antisera for Arthropoda 



This was a powerful antiserum producing well-marked results with 

 the Crustacean sera. Slight indications of a reaction were obtained 

 with two specimens of Reptilian, and two of Amphibian sera, probably 

 due to some fault in the dilutions (see pp. 211, 217, 310). 



(2) Tests with Anti-Grub Serum. 



This antiserum was obtained by the intravenous injection into a 

 Rabbit of the blood of a Crab (see pp. 211, 217, 310). 



Material tested 

 21 Egg-Albumins 



(a) 13 Birds' egg-albumins 13 



(6) 1 Beptile 1 



(c) 1 Amphibian 1 



(d) 6 Fish 6 



128 Blood Sera 



60 Keptilia 60 



18 Amphibia 18 



38 Pisces 38 



12 Arthropoda / * Xi P hosura 1 

 (11 Decapoda 



Percentage 

 of positive 



reactions 



o 





 

 





 

 

 

 100 



This was a very powerful antiserum giving well-marked reactions 

 with all specimens of the Crustacea. The serum of the King-crab 

 (Limulus polyphemus) failed to react. Tests with all other blood sera 

 and egg-albumins were negative. 



VII. Anti-Xiphosura Serum. 



% 



Tests with Anti- Limulus Serum, 



These tests were conducted with two different antisera for the serum 

 of the King-crab (Limulus polyphemus). The first was made by intra- 

 peritoneal, and the second by intravenous injections into Rabbits. In 

 the following table the tests made with both antisera are recorded. 



Both these antisera were extremely powerful, the first giving more 

 than three times as much precipitum as any other antiserum. The 

 second was not quite so powerful. The first antiserum produced a small 

 reaction with crab's serum and with spider serum (very dilute), and 

 three doubtful cloudings with fish, and two with Crustacean sera. The 



