Graham-Smith 361 



possible to repeat them, however, the results would probably not have 

 been materially affected. 



In the following tables the name of the collector of the serum is 

 given with the majority of specimens. Dr Nuttall acknowledges the 

 source of all specimens examined by him at the end of this book. 

 After all these samples the numbers given in Nuttall's tables are 

 quoted, so that the results obtained by him with a large number of 

 anti-Mammalian and anti- Avian sera may also be followed. 



The Limulus and Ammocoetes sera were procured for me by 

 Dr W. H. Gaskell, F.R.S., to whom I am much indebted for these very 

 interesting bloods. 



The previous work on these lines has already been fully considered 

 in the foregoing monograph, and for this reason no attempt has been 

 made here to summarize the results of other observers. 



The following works have been u.sed in the construction of the annexed tables : 

 EVANS, A. H. "Birds," Cambridge Natural History, Vol. ix. 1900. 

 GADOW, H. "Amphibia and Reptiles," Cambridge Natural History, Vol. vm. 1901. 

 GUNTHER, A. 0. L. (!. The Study of Fishes. 1880. 



General Conclusions. 



(1) Powerful antisera to Avian egg-albumins produce very large 

 reactions with dilutions of Birds' egg-albumins. They also give distinct 

 positive reactions with Chelonian and Crocodilian sera, as well as with 

 dilutions of Reptile egg-albumins, No reactions of any importance were 

 obtained with Amphibian or Fish egg-albumins or with Lacertilian, 

 Ophidian, Amphibian, Fish, or Crustacean sera. These tests, therefore, 

 show a distinct relationship between the Aves and Chelonia and 

 Crocodilia. 



(2) Powerful anti-Ghelonian sera gave maximum reactions with the 

 Chelonia and Crocodilia. Smaller reactions were obtained with Reptile 

 egg-albumins and Avian egg-albumins, but with Lacertilian and Ophidian 

 sera the results were almost negative. No positive reactions occurred 

 with other egg-albumins or blood sera. 



These results, showing a well-marked relationship between the Che- 

 lonia and Crocodilia, and distinct one with the Avcs, confirm the above 

 conclusion. 



(3) A moderately powerful anti- Crocodilian serum behaved like the 

 anti-Chelonian sera. 



