362 Conclusions 



(4) A powerful anti-Lacertilian serum produced its maximum 

 reactions with the Lacertilia, and also reacted well with the Ophidia. 

 Smaller reactions were obtained with the Crocodilia and Chelonia, but 

 none with the Avian egg-albumins or other albumins or blood sera. 



(5) A powerful anti-Ophidian serum gave well-marked results with 

 the Ophidia, and also reacted well with the Lacertilia. Reactions with 

 the Crocodilia and Chelonia were much smaller. A moderate reaction 

 was obtained with one Avian egg-albumin. All other tests were negative. 



(6) A very powerful antiserum to Ophidian egg-albumin showed its 

 maximum reactions with the Ophidia, good reactions with the Lacertilia, 

 and smaller ones with the Chelonia and Crocodilia. 36/o of distinct 

 positive reactions were obtained with Avian egg-albumins. Other tests 

 were negative. 



The results of experiments with anti-Lacertilian and anti-Ophidian 

 sera show a well-marked relationship between these two groups, a more 

 distant relationship between them and the Chelonia and Crocodilia, and 

 a still more distant one between them and the Aves. The latter is 

 most markedly shown by the anti-snake-egg serum. 



(7) , A moderately powerful anti-Frog serum reacted only with the 

 Anura. No reactions were obtained with the Urodela or any other 

 serum or egg-albumin tested. 



(8) A moderately powerful anti-Ammocoetes serum failed to show 

 any affinity between this animal and any other. 



(9) A \v<>ak (inti-Ascidian serum reacted only with its homologous 

 extract. 



(10) Two powerful anti-Crustacean (Decapoda) sera reacted well 

 with the sera of Decapoda, but failed to react with the serum of the 

 King-crab (Limulus polyphemus). Tests with egg-albumins and other 

 sera were all negative. 



(11) Two extremely powerful anti-Limulus sera reacted well with 

 dilutions of Limulus serum, and produced moderate reactions with spider 

 sera. One showed a small reaction with a crab serum, but the other 

 did not. Other tests with Crustacean sera were negative. Experiments 

 with Ammocoetes and other Vertebrate sera and egg-albumins were 

 negative. These tests, therefore, show a distinct relationship between 

 Limulus and the Arachnida, and a doubtful one between Limulus and 

 the Decapoda. 



