X CONTENTS. 



CHAPTER VI. 



PAGE 



Preparation ot the tubes, flasks, etc., in which the media are to 

 be preserved 109-112 



CHAPTER VII. 



Technique of making plates— Esmarch tubes, Petri plates, etc. . 113-124 



CHAPTER VIII. 



The incubating-oven— Gas-pressure regulator— Thermo-regulator — 

 Safety burner employed in heating the incubator 125-132 



CHAPTER IX. 



The study of colonies— Their naked-eye peculiarities and their ap- 

 pearance under different conditions— Differences in the structure 

 of colonies of different species of bacteria— Stab-cultures— Slant- 

 cultures 133-138 



CHAPTER X. 



Methods of staining— Solutions employed— Preparation and stain- 

 ing of cover-slips— Preparation of tissues for section-cutting— Stain- 

 ing of tissues— Special staining-methods 139-176 



CHAPTER XI. 



Systematic study of an organism— Points to be considered in iden- 

 tifying an organism as a definite species 177-205 



CHAPTER XII. 



Inoculation of animals— Subcutaneous inoculation ; intravenous 

 injection— Inoculation into the great serous cavities, and into the an- 

 terior chamber of the eye— Observation of animals after inoculation 206-227 



CHAPTER XIII. 



Post-mortem examination of animals— Bacteriological examina- 

 tion of the tissues— Dispoeal of tissues and disinfection of instru- 

 ments after the examination 228-233 



