CONTENTS 



INTKODUCTION. 



PAGE 



The overthrow of the doctrine of spontaneous generation—" Omne 

 vivum ex vivo"— Earlier bacteriological studies— The birth of mod- 

 ern bacteriology 13-26 



CHAPTEK I. 



De6nition of bacteria— Their place in nature— Difference between 

 parasites and saprophytes— Nutrition of bacteria— Products of bac- 

 teria—Their relation to oxygen— Influence of temperature upon 

 their growth 27-35 



CHAPTER II. 



Morphology of bacterial-Grouping— Mode of multiplication— 

 SjHjre-formation — Motility 36-46 



CHAPTER III. 



Principles of sterilization by heat— Methods employed— Discon- 

 tinued sterilization— Sterilization under pressure — Apparatus em- 

 ployed—Chemical disinfection and sterilization 47-71 



CHAPTER IV. 



Principles involved in the methods of isolation of bacteria in pure 

 culture by the plate method of Koch— Materials employed 72-78 



CHAPTER V. 



Preparation of nutrient media— Boaillon, gelatin, agar-agar, potato, 

 hlood-semm, etc 79-lOS 



