FILLING THE TUBES. Ill 



may be allowed to flow into the tubes held under its 

 opening. 



It is not necessary to sterilize the funnel^ for the 

 medium is to be subjected to this process as soon as it 

 is in the test>-tubes. 



Fig. 22. 



Fannel for filling tabes with cnltore media. 



Care should be taken that none of the medium is 

 dropped upon the mouth of the test-tube, otherwise the 

 cotton plug becomes adherent to it, and is not only 

 difficult to remove, but presents a very untidy appear- 

 ance, and interferes, indeed, with the proper manipula- 

 tions. 



As soon as the tubes have been filled they are to be 



