DESCRIBING AN ORGANISM. 205 



tions ? How much of this gas is carbonic acid and how 

 much is explosive ? 



12. At what temperature does it thrive best, and the 

 lowest and highest temperatures at which it will de- 

 velop ? AVhat is its thermal death-point, both by steam 

 and dry-air methods of determining this point ? 



13. What is its behavior when exposed to chemical 

 disinfectants and antiseptics ? Does it withstand dry- 

 ing and other injurious influences, lx)th in the vegeta- 

 tive and spore stages? The germicidal value of the 

 blood-serum of different animals may also be tried 

 upon it. 



14. Its pathogenic powers — modes of inoculation by 

 which these are demonstrated; quantity of material used 

 in inoculation; duration of the disease and its symp- 

 toms; lesions produced, and distribution of the bacteria 

 in the inoculated animal ; which animals are susceptible 

 and which immune, and the character of its pathogenic 

 activities? Variations in vimlence, and the probable 

 cause to which they are due. Can they be produced 

 artificially and at will ? 



15. The detection of specific, toxic, and immunizing 

 products of growth. 



16. Its behavior when exposed to the influence of 

 blood-serum of animals immunized from it; also its 

 behavior when mixed with serum from an animal in 

 the height of infection by it. Are the relations be- 

 tween the organism and the serum constant and spe- 

 cific? 



10 



