THE BACILLUS OF MALIGNANT (EDEMA. 443 



Fig. 95. 



When in pairs the ends that approximate are squarely 

 cut, while the distal extremities are rounded. When 

 occurring singly both ends are round- 

 ed. (How does it differ in this respect 

 from bacillus anthracisf) It is slowly 

 motile, and its flagella are located 

 both at the ends and along the sides 

 of the rod. It forms sjwres that are 

 usually located in or near the middle 

 of the body of the cell. These may 

 cause a swelling of the cell at the point 

 at which they are located and give to 

 it a more or less oval, spindle, or lozenge 

 shape. (Fig. 94, b.) 



It is a strict anaerobe, growing on 

 all the ordinary media, but not under 

 the access of oxygen. It grows well 

 in a hydrogen atmosphere. It causes 

 liquefaction of gelatin. 



In tubes containing about 20 to 30 

 c.c. of gelatin that has been liquefied, 

 inoculated with a small amount of the 

 culture, and then rapidly solidified in 

 ice-water, growth appears in the form 

 of isolated colonies at or near the bot- 

 tom of the tube in from two to three 

 days at 20° C. These colonies, when colonies of the ba- 



.", rv E- . I • T cillus of malignant 



of from 0.5 to 1 mm. m diameter, ap- oedema in deepgeia- 

 pear as little spheres filled with clear *^''- *^'^*"'^- (^^' 



|. - ^ Fkankkl and Pfkif- 



liquid, and are difficult, for this reason, fek.) 

 to detect. (Fig. 95.) 



As they gradually increase in size the contents of the 

 spheres become cloudy and are marked by fine radiating 



