274 



THE PATHOGENIC HEMOSPORIDIA 



In addition to the nucleus there appears to be a second brightly 

 staining structure in the cell, which Schaudinn ('04) first drew atten- 

 tion to from blood smears made by Kossel and Weber from cattle 

 with Texas fever, and regarded as a blepharoplast by Liihe, Nuttall 

 and Graham-Smith, Christophers, and others who have worked with 

 these forms. Nuttall and Graham-Smith, in addition, described a 

 third chromatin structure as a reticulate and faintly staining mass of 

 chromatin lying close to the nucleus, but Christophers and Kinoshita 

 give evidence to show that this is but a part of the nucleus (Fig. 108). 



Fig. 108 



z> 



H 



B 



C 



F 



Babesia (Piroplasma) canis. (After Christophers.) Different stages in the erythrocyte 

 culture media, and, /, in embryonic salivary cell of tick Rhipicephalus sanguineus. 



A number of investigators have attempted to cultivate babesia on 

 artificial culture media, a limited success only being obtained, the chief 

 result being merely the prolongation of life of the parasites in the 

 infected blood kept under proper conditions. In this way Chris- 

 tophers and others obtained various morphological changes in the 

 organisms, but no developmental processes. Kleine ('00) and Miya- 

 jima ('07), on the other hand, claim to have produced many develop- 

 mental stages in vitro, the former with Babesia canis in young dog's 



