23 



Cut out a small piece with the scissors and spread it out in a 

 thin layer on the slide, using a dry slide and new needles. Draw 

 the edges away from the middle, and if they are dry enough they 

 will stick to the slide and hold the film outspread. It will probably 

 be necessary to absorb some of the liquid by means of lens paper be- 

 fore you can make the edges stick. Do not allow the middle to dry. 

 Place on the middle a drop of normal salt solution and a cover-glass. 



Examine the preparation carefully, recognizing (#) the white 

 fibers, in wavy bundles ; () the yellow elastic fibers, straight and 

 single ; if possible find a place where they anastomose (see, how- 

 ever, below) ; ( the connective tissue cells ; can you find more 

 than one kind? (See 22). 



Action of Reagents. Remove the cover glass and place upon 

 the preparation a drop of 2% acetic acid. Note that it makes some 

 of the fibers disappear, the white fibers, while others, the yellow 

 elastic, are sharply brought out. It is now possible to observe the 

 course, anastomosing and branching of the yellow fibres. Note 

 also that the nuclei of the connective tissue cells are more sharply 

 defined. 



22. Subcutaneous areolar tissue. Stained preparation. 

 Assigned for study. Tissue spread on the slide by means of 

 needles, as in 21 ; stained with hematoxylin and erythrosin (or 

 eosin) ( 79). 



Study the preparation carefully, recognizing again the white 

 and yellow fibers and the cells, of which find if possible three 

 kinds (text-book). 



23. Adipose tissue. Demonstration. Spread preparation. 

 Stained with hematoxylin and eosin ( 79). 



In this preparation of subcutaneous adipose tissue note (a) the 

 lobular grouping of the fat cells, (^) the blood vessels and the rela- 

 tion of the fat cells to the capillary network, (<:) the structure of 

 the individual fat cells, the fat globule, the nucleus with the proto- 

 plasm surrounding it. 



WHITE FIBROUS TISSUE. 



24. Tendon. From the tail of a mouse. Tendons pulled 

 out, spread out flat by means of a needle and stained 15-20 minutes 



