24 



with Khrlich's acid hematoxylin ( 81), now in glycerin. Mount in 

 glycerin jelly ( 105). 



To show the tendon cells (connective tissue cells). Note their 

 arrangement in parallel rows between the tendon fibers, their shape 

 in relation to each other, the position of the nucleus in the cell in 

 relation to the nucleus of the contiguous cell. Consult also 26 for 

 the true shape of the cells. 



25. Tendon. From the tail of a mouse. Silvered to show neg- 

 ative images of the tendon cells ( 147). The tendons were pulled 

 out and treated for a few minutes with a solution of silver nitrate, 

 washed in water, exposed to the light, washed in water ; now in 

 glycerin. Mount in glycerin-jelly. The places occupied by the 

 cells are white, while the cement substance was colored brown by 

 the silver nitrate on exposure to the light. Note the outlines of the 

 cells and compare with the positive images of 24. 



26. Tendon. Transection of tendon, biceps of a new-born 

 puppy. Miiller's fluid ( 29) ; paraffin ; sections yw. 



Stain 30 minutes with hematoxylin, 15 seconds with picro- 

 fuchsin. Mount in balsam. Note the relation of tendon-cells and 

 fibers as shown in transection and compare with 24 and 25. Note 

 also the staining reactions of the white fibrous tissue with picro- 

 fuchsin in comparison with the transected muscle fibers (central), 

 and the elastic tissue in 28. 



27. Cornea. Cat. Assigned for study. Free-hand sections. 

 The cornea silvered ( 147), free-hand sections cut from the ental 

 surface and mounted in glycerin jelly. v < 



To show the negative images of the cells of dense connective 

 tissue (i. e., the cornea). Note the shape of the cell spaces, and the 

 anastomosing of the processes ; compare with the similar preparation 

 showing the cell spaces in tendon, 25. 



YELLOW ELASTIC TISSUE. 



28. Ligamentum nuchae. Horse. Transection. ; 

 paraffin ; sections yw. Stained with hematoxylin and picro- 

 fuchsin, now in 95% alcohol. Clear and mount in balsam. 



Note the yellow elastic fibers, their shape and size in transec- 

 tion, the white connective tissue in between them, and the nucleus 



