2 9 



osteum. Study carefully the centers of ossification in the epiphysis 

 and shaft, so as to understand the dissolution of the cartilage (de- 

 generation of the cells, absorption of the matrix) and the deposit of 

 bone. How is spongy bone converted into dense bone ? Study the 

 structure of the periosteum, recognizing the two layers ; understand 

 the part it plays in the processes of ossification. In addition, study 

 the preparation for the structure of cartilage, articular cartilage, the 

 general structure and articulation of a long bone^ 



39. Transaction of a long bone. VvA?V*^ JUA^/WMJ^-O Picric 

 alcohol; decalcified in nitric acid decalcifier ( 131) ; collodion ;. 

 sections CLi* //. Stain in hematoxylin bO minutes, 



seconds. Mount in balsam. 



Study, noting (a) the marrow with its nutrient artery, () the 

 structure of the bone, (c) the structure of the periosteum. Com- 

 pare the structure of the decalcified bone with the dry preparation 

 of bone, 36. Recognize the identity of structure in the two prepar- 

 ations so differently prepared. 



TOPICS FOR QUIZ 7th Week. 



THE CONNECTIVE AND SUPPORTING TISSUES CONTINUED. 



9. Cartilage, general character ; varieties, and the situation of each? 

 10. Embryonal cartilage. When found and where situated? 

 ir. Hyalin cartilage, character and distribution? 



12. Elastic cartilage, character and distribution? 



13. Fibro-cartilage, character and distribution? 



14. Bone, the varieties, structure and situation ? 



15. Development of bone. Be ready to give the steps as described in 

 Piersol. 



1 6. Centers of ossification ? 



17. Compare the intercellular or ground substance of bone and cartilage 

 with that of the other connective tissues. 



