33 



DEVELOPING RED BI,OOD CORPUSCLES. 



48. Smear preparation of red marrow. 



A drop or red marrow was spread upon a cover-glass as a thin film 

 ( 12 1 ) ; fixed in a saturated solution of mercuric chlorid ( 26) 

 15 minutes or so, and placed in 67% alcohol. Rinse off the alcohol 

 with distilled water and stain with Bhrlich's triacid mixture ( 125) 



ifWi 



for 10-15 minutes, rinse with distilled water, dry and mount in - 

 balsam. 



In this preparation find () marrow cells, medium sized cells 

 with a large nucleus and scanty protoplasm, resembling leucocytes 

 somewhat ; (^) giant cells, with one or many nuclei ; (e) cells with 

 deeply staining nuclei and more or less hemoglobin in the cell body 

 (stained orange), erythroblasts ; (d) finally, red blood corpuscles, 

 non-nucleated. Find as many forms of erythroblasts as possible, 

 and compare, with the red corpuscles. 



TOPICS FOR QUIZ 8th Week. 



THE BlyOOD AND THE IvYMPH. 



1. What and where is blood, (a) plasma or intercellular substance; (b) 

 corpuscles ? 



2. What and where is lymph, (a) plasma ; (b) corpuscles? 



3. Character of the corpuscles in the vertebrates ? 



4. Size of the corpuscles in man, horse, ox, dog, necturus? 



5. Methods of study ; moist, dry and stained preparations. 



6. Compare the blood corpuscles of man, rabbit, camel, lamprey and 

 necturus. 



7. Action of reagents on the corpuscles? 



8. Development of the corpuscles in the embryo and in the adult? 



