Paraffin or Collodion Sections 

 (66 and 67) 



v 

 Benzin 



95 % Alcohol 



Water 



v 

 Hematoxylin, 10-30 min. 



v 

 Water 



v 



Picrofuchsin. eosin 

 or erythrosin, ^2-2 min. 



v 

 Distilled water or 67 % alcohol 



v 

 Dehydrate (95-99 % alcohol) 



Clear 



(carbol-xylene) 



y 

 Mount in Balsam 



STAINS. 



| 80. Chloral Hematoxylin. (Proc. Am. Micr. Soc., vol. XIV, pp. 125- 

 127). Formula: (A) Hematoxylin crystals, -fa gram, dissolved in 10 c.c. of 

 95% alcohol ; (B) distilled water, 100 c.c., potash alum, 3^ grams ; boil for 5 

 minutes in an agate-ware or glass vessel and add sufficient boiled water to bring 

 the volume back to 100 c.c. When cool, mix (A) and (B) and add chloral hy- 

 drate, 2 grams. Allow the solution to ripen for a week or two in the air and 

 sunlight, or add 1-2 c.c. hydrogen dioxid, when it is ready for use. Its staining 

 quality improves up to an optimum and then begins to deteriorate. Old 

 hematoxylin generally contains a precipitate and should be filtered often or 

 before using. 



Stain sections from water for 5.30 minutes according to the age of the solu- 

 tion, the character of the tissue and the fixation employed. After staining, 



