Iron Hematoxylin 



Any Fixation desired 



v 

 Paraffin Sections 



v 

 Benzin 



95.% Alcohol 





Water 



I 



v 



Mordant 

 1-3 hours in 4 % ferric alum 



Wa V ter 



v 



Stain 1-6 hours 

 in V<2 % aq. hematoxylin 



Water 



V 



Differentiate, by dipping alternately in 



mordant and in the tap-water, 



a second in each 



I 



v 



Water (running) 

 15 minutes 

 I or 



v 



Orange G. 

 sat. aq. sol.) 



v 

 Water (rinsed) 



> 95-99 % Alcohol 



v 

 Clear in Carbol-xylene 



I 



v 

 Mount in Balsam 



