79 



cation of the salt so much heat is evolved that the temperature 

 in. the inner container keeps fairly constant for several hours. The 

 same result could obviously be obtained . with water instead of 

 zinc nitrate but a much larger quantity would have to be used which 

 would make the apparatus considerably heavier and larger.* 



In this incubator the inoculated swab was conveyed to the Serum 

 Institute where cultures were made about an hour after the sample 

 was made. Water-agar plates were not used, but the glass rod was 

 rubbed several times over the infected cotton wool swab and then 

 rubbed on the surface of the nutritive medium. 



In the table below, „0D",. „1D", and „2D" signify that 

 the sample was taken the same day, the first day and the 

 second day respectively, after the commencement of the disease. 

 The occurrence of Pfeiffer's bacillus is denoted by: „0" = ba- 

 cillus not found; „I" = only a few colonies; „H" .-=» rather 

 numerous colonies, but only constituting a small proportion 

 of the total number of colonies; „III" = colonies of Pfeiffer's 

 bacillus at least as numerous as the other colonies; „IV' W " = 

 practically a pure culture of Pfeiffer's bacillus. 



D. 



ID. 10 1 



2D. 4 



Just as in 1918 — 19 Pfeiffer's bacillus was found most con- 

 stantly at the beginning of the disease, but it was not the 

 rule that it disappeared very quickly. Altogether it was found 

 in 24 (63o/o-) of the 38 soldiers. 



In a large number of the examinations of influenza patients 

 the microbe was verified by all the tests referred to on pp 68—70 

 (for the demonstration of haemoglobinophilia only ascitic agar, not 

 ordinary agar, was used as a rule). As however it soon became 

 evident that the colonies in the primary culture on haemoglobin 

 agar were of such a characteristic appearance that they could be 

 detected with great certainty by microscopic observation I have 

 in a number of cases been satisfied with this in connection with 

 the microscopic examination. There is no reason to believe that 

 this abbreviated examination has lead to any error.** 



* I have, however, later realized that is very doubtful if the 

 transport by elevated temperature is advantageous. 

 ** Pfeiffer's bacilli from other than influenza patients were always 

 verified by a complete examination. 



