89 



1920 (see Thomsen & Wulff) in the course of 3 days, from 

 the entire personel, in all, 225 men (recruits who had been there 

 for about I1/2 months). It was the original intention only 

 to search for Meningococcus for which reason only ascitic 

 agar was used for cultivation. This proved to contain a little 

 blood *, as a plenty growth of Pfeiffer's bacilli appeared on a 

 large number of the plates. I seized the opportunity of exa- 

 mining 165 of the plates more closely (the rest which referred 

 to the first day, were rejected at" that date). In 54 (33%) of 

 the plates Pfeiffer's bacilli were found, typical in each case. (By 

 using haemoglobin-agar instead of ascitic agar, a rather higher 

 figure would probably have been obtained, by including some 

 more of the specially fastidious atypical Pfeiffer's bacilli). 



On 6. IX, 7. IX. and 10. IX. 21. inoculations from 452 

 recruits in the Vserlose camp who had been there from' 25. 

 IV. were made. The inoculated swabs were conveyed in test 

 tubes just as in the case of influenza in January 1922. They 

 were however carried in the more primitive „hay -box", in which 

 the temperature on reception at the Institute was 22 — 24°. 

 The lapse of time between the taking of the samples and the 

 inoculation of the plates was li/ 2 — 21/2 hours. 



In this and the following investigations agar with dissol- 

 ved haemoglobin was not employed, but in place of it, blood- 

 agar. (The mixture of melted agar and blood was heated 

 at 50°— 60° until it acquired a dark red colour. On such 

 slightly healed blood-agar Pfeiffer's bacillus grows more 

 vigorously than on ordinary blood-agar). 



Pfeiffer's bacillus was found in 63 persons (14<y ); 46 were 

 typical and 17 atypical. 



On 17. IX. 1921 inoculations were made from 230 recruits 

 in the Jaegersborg camp (the same camp as that in which the 

 investigations of July 1920 were carried out). They had been 

 on duty from 25. V. The technique was the same as that used 

 in the Vserlose camp. Pfeiffer's bacillus was found in 78 men 

 (34 0/0); 65 were typical and 18 atypical. 



It must be mentioned that in both the last-named investiga- 

 tions, Pneumococcus, Meningococcus and the Diphtheria bacil- 

 lus were also looked for. No relation could be demonstrated 



Which could also be demonstrated by the benzidin test. 



