109 



On 9. IX. 1920 the following strains were inoculated on ordi- 

 nary and on ascitic agar: I 1 (a), 1 (b), 5, 6, 20, 21, 22 fa), 22 (b), 

 23, 27, 29, 35, 38, 45, 54, 57 (a), 57 (b), 71, 72, 91 (a), 91 (b), 100^ 

 103; I 5, 21, 32, 33, 34, 35, 36, 37; P 14, 15 (a), 15 (b), 22, 23, 24, 25, 

 26; Ms 1, 4, 9, 10, 13, 15; Me 1; H 14, 19 (a), 19 (b), 34, 35a, 37a, 

 37c, 49b, 122, 127, 133, 151, 152 (a), 152 (b), 154, 155, 156, 157, 158, 

 159, 160, 164, 168, 171, 172, 176, 178, 179, 181, 197, 205, 260, 261, 

 263, 264, 265, 266, 268-81, 283, 284, 286-89, 291, 293-296, 298-304, 

 306-313; Pa 4, 5. 



The agar reacted strongly alkaline to azolitmin paper but did 

 not react to a-naphthol-phthalein (see p. 226). To control the value 

 of the agar as a nutritive medium, a very small amount of haemo- 

 globin (dissolved blood corpuscles) was added to some of it. Plates 

 of this were inoculated with various strains of Pfeiffer's bacilli together 

 with the ,, white air coccus" which produced a very rich growth 

 of the Pfeiffer's bacilli. 



The ascitic agar was prepared from the same agar and the ascitic 

 fluid ,,1,023". Hydrochloric acid was added till the reaction became 

 weakly alkaline to azolitmin paper. As a control of the efficiency 

 of the medium a plate was inoculated with 2 cultures of Gonococ- 

 Cits which gave a good growth and 3 cultures of the whooping- 

 cough bacillus which gave a medium growth, while on the agar 

 without ascitic fluid one Gonococcus cultivated grew moderately, 

 the other and also the whooping-cough bacillus cultures, not at all. 



The agar and ascitic agar were inoculated from cultivations 

 of all the strains of Pfeiffer's bacilli mentioned on FiLDES-agar (see 

 p. 229), streak inoculations of these being alternated with streaks 

 of the „while coccus". 



After 4 days in the incubator there was no trace of growth 

 on the agar plates. 



The following strains also did not grow on the ascitic agar 

 plates- I 6, 38, 45, 54, 57, 71; I 32, 33, 34; Ms 1; H 14, 19 (a), 

 35 (a), 122, 152 (a), 152 (b), 158, 160, 171, 176, 181, 205, 269, 272, 

 291, 295, 303, 304, 306, 307, 312. The remaining strains grew more or 

 less Iuxurianlly on the ascitic agar plates but exclusively in the 

 immediate neighbourhood of the coccus colonies. At a distance from 

 these there was no growth in any of the cases. All the strains 

 which grew were again inoculated on the same medium, always in 

 association with the „air coccus". During the course of the ex- 

 periments freshly prepared ascitic agar plates were required; they 

 were controlled by the previously mentioned method. With each 

 inoculation the plates were examined after 4 or 5 days incubation. 



In the inoculations on ascitic agar in the second generation, 

 two cultures were made from each strain. It should be noted that 

 the results of these parallel tests diverge considerably from one 

 another. From all the strains which grew cultures were again made, 

 which represented the 3rd. generation, and the following strains 

 showed growth but only around the coccus colonies: I 1 (a, b), 5, 



