110 



35, 57, 59; P 15 (a, b), 23; Me 1 a, b, c, d; H 34, 157, 159, 280, 

 183, 284, 286, 289, 302, 308, 309, 310, 311, 313. But I 36 and H 155 

 grow particularly well and there was also distinct grovvlh outside 

 the sphere of influence of the „air coccus". In the next subculture 

 these two strains still grew in the same manner. 



Hnally on 1. XI. 1921 the following strains were inoculated 

 on ordinary and ascitic agar: I 1 (a, b), 5, 6, 20, 21, 22 (a, b), 23, 

 27, 29, 35, 38, 57 (a, b), 59, 71, 72, 91 (a, b), 100, 103; I 5, 21, 

 32, 33, 34, 36, 37; P 14, 22-25; Ms 9, 13, 15; Me la, b, 2, 3, 4, 5; 

 H 19, 34, 49b. 122, 127, 133, 151, 152 (a, b), 154, 155, 156, 157, 159, 

 164, 168, 176, 179, 181, 197, 205, 260, 261, 263-236, 268. 269, 272, 274, 

 276, 280, 287,' 288, 289, 291, 293-296, 298, 299, 300, 302, 305, 307—312, 

 314 a, b, c, d, 318, 319, 320, 322-325, 327-339. 341-350, 352; GP 1, 

 2, 3, 4 a, b, 5; Pa 4, 5; R 1, 2. 



In the preparation of the ascitic agar the fluid ,,1,023" was used. 

 The finished medium reacted slightly alkaline to azolitmin paper, 

 and 4 strains of whooping-cough bacillus which did not grow on 

 agar without ascitic fluid, grew well on it. The ascitic agar was 

 used in a freshly prepared condition. A number of the strains were 

 also inoculated on ascitic agar plates which had been incubated 

 for 24 hours, but this made no difference. 



The following strains grew slightly in the 1st. culture on or- 

 dinary agar: H 314 a, b, c, d, 318, 319, 322, 321, 330; GP 1, 2, 3, 4b; 

 but in no case was there growth in the 2nd. culture. On ascitic 

 agar most of the cultures gave no growth evien in the 1st. culture. 

 In the 2nd. culture the following showed slight growth: H 289, 314b, 

 318, 319; Pa 4. 



Of the two strains which succeeded in growing in pure culture 

 on ascitic agar in the experiment on 9. IX. 1920, I 36 did not 

 grow in the 1st. culture, and H 155 only grew slightly. 



It appears from this experiment that even those strains 

 that had been cultivated for over three years, during which time 

 they had been subcultured more than 200 times, — on medium 

 rich in haemoglobin it is true, — had retained their dependence 

 on haemoglobin unchanged. 



We might imagine that it would perhaps be possihle by gra- 

 dually reducing the haemoglobin content of the medium, lo train 

 Pfeiffer's bacilli to be satisfied with a smaller amount and finally 

 to do entirely without it. Apropos of this some experiments will 

 be mentioned which partly deal with this question and partly give 

 some information about growth in different concentrations of hae- 

 moglobin. 



In August 1918 plates were prepared containing dissolved blood 

 corpuscles in roughly the following concentrations (calculated as 

 blood corpuscles, not haemoglobin): 1 : 10, 16, 30, 50, 80, 120, 200, 



