113 



(1). Observed twice. 



(2). Slight growth after 4 days. 



(3). In many cases no visible growth after 2 days, but distinct 



growth after 4 days. 

 (4). (-J-) signifies that there was growth only in one of the 



two plates constituting each test. 

 (5). In the next subculture (4th. culture) there was no growth or 



only a trace, in each case. 



1 

 The cultivation was continued on the plates containing Viooo 



blood corpuscles to the 5th. or 6th. generation without any sign 



of adjustment to the new conditions. On the contrary the growth 



gradually diminished. 



A number of strains (50 in all) were inoculated on ordinary broth 



in test tubes. In no case was there any visible growth. 



The object of performing the experiments of inoculation 

 on haemoglobin-free media in such large number and of repor- 

 ting them so minutely, is to bring forward two points: (1) 

 whether distinct individual differences between the various 

 strains can be detected with regard to their dependence on 

 haemoglobin, and (2) whether haemoglobinophilia can be re- 

 garded as a constant character. i 



As regards the first point there seem' to be certain differen- 

 ces of such a kind that some strains can be accommodated with 

 less haemoglobin than others, but nothing definite can be stated 

 about this question, and large, well j marked differences at any 

 rate, do not exist. 



8 



