370 ■ METHODS. 



5. Wash thoroughly in water. 



6. Decolorize in the iron solution ; the section must be gently moved about 

 to secure an even decolorization. When part of the section shows a brown color, 

 it^should be removed and examined. 



7. Wash thoroughly in water to remove the iron solution, no trace of which 

 can be left without ruining the specimert. 



8. Dehydrate with alcohol and mount at once in dammar. 



7. Mai.i.ory's Tripi^e Connective-tissue Stain. 



Formula I : Acid fuchsine, i.o gm. 



Distilled water, looo.o c.c. 



II: Phosphomolybdic acid, 1.0 gm. 



Distilled water loo.o c.c. 



Ill: Aniline blue, soluble in water, 0.5 gm. 



Orange G, 2.0 gm. 



Oxalic acid, 2.0 gm. 



Distilled water, loo.o c.c. 



1. Preserve in corrosive sublimate or Zenker's fluid. 



2. Stain the sections in the fuchsine solution one to three minutes. 



3. Wash in water, 



4. Place in the phosphomolybdic solution one minute. 



5. Wash in two changes of water. 



6. Stain in blue and orange solution two to twenty minutes. 



7. Wash in water, 



8. Dehydrate in 95 per cent, alcohol and mount in dammar. 



This method gives a perfect differential stain of connective- tissue fibrils, 

 and it is to be used whenever the fibrils are to be especially studied. 



