THE MANUFACTURE OF HEMOGLOBIN 



71 



retain a great part of their haemoglobin. If, instead of a microscopic 

 preparation, a little of the original dialysed "rather transparent" 

 solution be taken in a test-tube and some salt added, it immediately 

 assumes the appearance of normal blood. 



Clearly the solutions with which we had been working were not 

 solutions in the accepted sense at all. They consisted of a mass of 

 corpuscles which as dialysis had proceeded gradually swelled up at 

 the expense of the surrounding fluid till they finally almost lost their 

 refrangibility. Consequently it became possible to see light through 

 the solution as is seen through corpuscles which are pressed together 

 in a hsematocrit. It now seemed that a possible explanation of the 

 irregular behaviour of our solutions was forthcoming. The dissociation 

 curve of haemoglobin even within the saltless corpuscle might be 

 essentially different from that of free haemoglobin in a correspondingly 

 salt-free solution. 



The above observations have been expanded by Brown, and indeed 

 a whole literature has grown up around the subject, but it takes us 

 away from the manufacture of haemoglobin and to the subject of 

 reversible haemolysis (ii), (i2). 



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