90 HEMOGLOBIN 



The results may be expressed in terms of the value of n, the 

 calculated number by which 16,700 must be multiplied to give the 

 true molecular weight. ' 



Value of n 

 COHb Met-Hb 



Number of Series I Series 11 Series I Series II 



experiments ... 9 10 8 12 



Maximum ... 4-59 4-58 4-56 407 



Minimum ... 3-49 3-98 3-79 3-19 



Mean 406 4-25 4-18 3-73 



Considering the amazing boldness of the technique it will be agreed 

 that these experiments leave no doubt that in the strength of solution 

 used the value of n is 4. The results of Adair and Svedberg are 

 therefore in exact agreement. 



There is one more thing to be said : a given value of n might merely 

 be the average of a widely distributed range of values of n for different 

 aggregates. Adair's results rendered this improbable. According to 

 Svedberg's, there was no suggestion of differential ultracentrifu- 

 galisation in his tubes. Svedberg and Nichols report that they have 

 also confirmed Adair's determination which showed that n was 

 constant over a considerable range of hydrogen-ion concentration (9), 



REFERENCES 



(1) Httfner and Gansser. Arch. f. Anat. u. Physiol, p. 209. 1907. 



(2) Reid. Journ. Physiol, xxxni. 12. 1905. 



(3) RoAF. Journ. Physiol, xxxvni. i (Proceedings). 1909. 



Quart. Journ. Exper. Physiol, rn. 75. 1910. 



(4) Adatr. Proc. Roy. Soc. A. cvm. 627. 1925. 



(5) Adair. Proc. Roy. Soc. A. cix. 292. 1925. 



(6) Adair. Proc. Camb. Phil. Soc. Biol. i. 75. 1924. 



(7) Adair quoted by Barcroft. Journ. Chem. Soc. p. 1146. May, 1926. 

 [For other Adair references, see Chap, i.] 



(8) Svedberg. Journ. Amer. Chem. Soc. xLvni. 430. 1926. 

 [For other Svedberg references, see Chap, i.] 



(9) Svedberg and Nichols. Journ. Amer. Chem. Soc. xux. 2920. 1927. 



