CARBON MONOXIDE— REDUCED HEMOGLOBIN 



159 



Having overcome the difficulties of estimation there remain others 

 of a much more subtle character. Of these one was pointed out by 

 Douglas, Haldane and Haldane(i) in the following words: "Violent 

 shaking had to be avoided on account of its effect in producing 

 mechanical coagulation, but satisfactory saturation could easily be 



Fig. 51. Ordinates = percentage saturation of haemoglobin with oxygen. Abscissae = 

 tension of oxygen in mm. of mercury. Curve I = rectangular hyperbola. 

 XY = 800. Curve II = Bohr's dissociation curve of haemoglobin. 

 © Points determined from dialysed solution. 

 D Points determined from undialysed solution. 



obtained by rotating the blood in a saturator." Now the pressures 

 of carbon monoxide with which Douglas, Haldane and Haldane 

 worked were measured in tenths of a milUmetre, those with which 

 Hecht and Morgan worked were measured in hundredths or thou- 

 sandths of a millimetre, therefore the latter workers could not avoid 

 violent shaking, but by buffering the solution weU on the alkaUne 



