EFFECT OF TEMPERATURE ON HEMOGLOBIN 179 



constant in Hill's equation is the reciprocal of this) it is possible to 

 work out from them the temperature coefficient of the reactions. 

 This is done most simply by plotting the logarithms of the 50/50 

 pressures against the reciprocal of the absolute temperatures. The 

 result is given in Fig. 58. 



1-6 



1-4 



1.2 



1-0 





 '0032 



0033 



■0034 



'0035 



Fig. 58. 



Ordinate 

 Abscissa = 



= logarithm of pressure giving half-saturation. 

 ■■ reciprocal of absolute temperature. 



Over a range of ten degrees, between 17° C. and 27° C, the tempera- 

 ture coefficient is 3-2 and between 27° C. and 37° C. the temperature 

 coefficient is 2*8. At this distance of time I cannot remember, if 

 indeed I knew, what species of animal provided the blood in question, 

 but the result shows a temperature coefficient of rather less, though 

 not much less, than Hartridge and Roughton(8) found for the sheep 

 by kinetic methods. 



