232 APPENDIX 



bination is then turned around, and the flies fly into 

 the smaller bottle or the smaller bottle may be held 

 firmly underneath the other and the flies shaken into 

 it by jarring. The small bottle can then be plugged, 

 and a bit of cotton with four or five drops of ether 

 put into it. In a minute or two the flies are under 

 the influence of the ether and may be emptied out 

 on to a piece of white paper, or a white glass plate. 

 Some workers prefer to have the small bottle already 

 saturated with ether before the flies are shaken into 

 it; in this case they become etherized almost imme- 

 diately. They can then be examined with a hand 

 lens or with a binocular microscope. Some of the 

 characters require for study the latter or an ordinary 

 microscope. 



With a camel's hair brush the flies are pushed out 

 into a row and then sorted out, from right to left, 

 into an upper and a lower row, each of which may 

 again be subdivided. If overetherized, the wings 

 stand out above and at right angles to the body. If 

 insufficiently etherized, so that they recover before 

 they can be examined, they may be etherized again. 



The pure stock is kept in pint bottles and new 

 cultures made up each week. Descriptions of the 

 mutants as far as published will be found in the fol- 

 lowing journals: 



BAR. "A New Sex-linked Character in Drosophila." Biol. Bull., 



XXVI. 1914. 

 BEADED. "The Analysis of a Case of Continuous Variation, Etc." 



Amer. Nat., XLIII. 1914. 

 BENT. "A Gene for the Fourth Chromosome of Drosophila." 



Jour. Exper. Zoo/., XVII. 1914. 



