ACUTE INTOXICATION RABBITS. 19 



SERIES A. 

 (Doses of 147 to 167 mg of caflein per kilo were employed in these experiments.] 



Rabbit $32. Belgian hare, female. Weight, 1,070 grains. Diet, oats. 



March 25: 8.5 cc 2 per cent caffein (158 mg per kilo) injected subcutaneously at 

 2.15 p. m.; 4 p. m., reflexes increased; 5.45 p. m., increases of reflexes still more 

 marked . 



March 26: Rabbit looked normal; no symptoms observed. 

 Rabbit SSI. Belgian hare , female. Weight, 1,170 grams. Diet, oats. 



March 25: 2.15 p. m., 9 cc 2 per cent caffein (153 mg per kilo) injected subcutane- 

 ously; 4 p. m., reflexes increased; 5.45 p. m., condition the same. 



March 26: Rabbit looks normal; no symptoms observed. 

 Rabbit 328. Belgian hare, female. Weight, 1,200 grams. Diet, oats. 



March 25: 9 cc 2 per cent caffein injected subcutaneously (150 mg per kilo); 4 p. m., 

 reflexes increased; 5.45 p. m., reflexes increased but not markedly. 



March 26: No symptoms; rabbit looks normal. 

 Rabbit 322. White female. Weight, 1,065 grams. Diet, oats. 



March 17: 8 cc 2 per cent caffein (150 mg per kilo) injected subcutaneously at 11.55 

 a. m.; 12.55 p. m., reflexes increased, but no tetanus nor any other symptoms. 



March 18: Rabbit running around in cage; condition apparently normal. 



March 25: Condition of rabbit good. 

 Rabbit 217. White. Weight, 1,355 grams. Diet, oats. 



October 29: 10 cc 2 per cent caffein (147 mg per kilo) injected subcutaneously at 

 1.51 p. m. 5.15 p. m., rabbit alive; survived. 

 Rabbit 219. Maltese. Weight, 1,820 grams. Diet, oats. 



October 29: 14 cc 2 per cent caffein injected subcutaneously at 1.40 p. m. (153 mg 

 per kilo); 5.15, rabbit alive; survived. 

 Rabbit 194. White female. Weight, 1,490 grams. Diet, oats. 



October 14: 13 cc 2 per cent caffein (174 mg per kilo) injected subcutaneously; 

 increased reflexes and tremors were observed. 



October 15: Condition of rabbit good; no symptoms. 

 Rabbit 191. Brown male. Weight, 1,915 grams. Diet, oats. 



October 14: 16 cc 2 per cent caffein (167 mg per kilo) injected subcutaneously; 

 reflexes increased and tremors present. 



October 15: Condition of rabbit good. 



A study of this series shows that about 150 mg of caffein per kilo caused increased 

 reflexes within one to two hours after injection. When the dose was increased, as 

 in rabbits 194 and 191, the symptoms were more pronounced; 150 mg per kilo may 

 be regarded as the minimum dose which produces symptoms of nervous irritability 

 when caffein is injected subcutaneously. Experiments with larger doses were there- 

 fore carried out in order to determine the minimum fatal dose. 



SERIES B. 



Approximately 0.2 gram of caffein per kilo was employed in these experiments. 

 Diet and race as possible factors which may influence the toxicity of caffein were 

 made the subject of study in these experiments which were divided into two groups 

 as shown in the table, page 25. 



Rabbit 95. Gray and whit* male. Weight, 1,478 grams. Diet, oats. 



February 27: 11.30 a. m., 15 cc 2 per cent caffein (210 mg per kilo) injected sub- 

 cutaneously; 2.20 p. m., no symptcms, tremors observed when handled, but not 

 marked, reflexes slightly increased, no muscular rigidity nor any other symptoms; 

 2.45 p. m., rabbit suddenly became very restless, jumped off the table, and had 

 convulsions; 3.45 p. m., rabbit died, rigor mortis set in almost immediately after 

 death. 



Rabbit 96. Gray and white male. Weight, 1,585 grams. Diet, oats. 



February 27: 16 cc 2 per cent caffein (200 mg per kilo) injected subcutaneously 

 at 3.40 p. m.; increased reflexes observed about one hour after caffein was injected, 

 but no other symptoms. 



February 28: Rabbit found dead. 

 Rabbit 112. Black female. Weight, 875 grams. Diet, oats. 



March 18: 9 cc 2 per cent caffein (205 mg per kilo) injected subcutaneously at 

 3 p. m.; 3.30 p. m., rabbit became restless, reflexes were increased, tremors were 



