ACUTE INTOXICATION RABBITS. 27 



Ifnbbit 249. Belgian hare. Weight, 890 grams. Diet, oats. 



November 17: 1.30 p. m. f 14.5 cc 2 per cent caffein (325 mg per kilo) administered; 

 4.30 p. in., hyper-sensitiveness; no other symptoms. 



November 18: 10 a. m., no symptoms; urine collected, iu> reduction; rabbit sur- 

 vived. 



SERIES B. 



The object of these experiments was to determine the minimum fatal dose of caffein 

 in the two varieties of rabbits, the white and the gray. All of the animals selected 

 were approximately of the same weight. 



Rabbit 289. Belgian hare, male. Weight, 935 grams. Diet, oats. 



November 19: 4 p. m., 17 cc 2 per cent caffein (363 mg per kilo) administered by 

 mouth, followed by 10 cc of 0.9 per cent salt solution. 



November 20: Urine examined, no sugar found, no symptom noticed at any time 

 after injection. 

 Rabbit 254. Belgian hare, female. Weight, 975 grams. Diet, oats. 



November 19: 4.05 p. m., 18 cc 2 per cent caffein (369 mg per kilo) administered by 

 mouth, followed by 10 cc of 0.9 per cent salt solution. 



November 20: 9 a. m., rabbit found dead. 

 Rabbit 267. White. Weight, 1,050 grams. Diet, oats. 



November 23: 12.10 p. m., 18 cc 2 per cent caffein (342 mg per kilo) given by mouth, 

 followed by 18 cc salt solution; 1 p. m., increased reflexes, tremors marked but no teta- 

 nus; 1.05 p. m., rabbit stretched on abdomen, posterior extremities in extended position 

 and paralyzed, soon after clonic spasms set in, which recurred about every minute; 

 1.14 p. m., tetanus and death. Autopsy: Liver showed fatty degeneration; slight in- 

 flammation of stomach and intestines; other organs normal. 

 Rabbit 268. White. Weight 1,100 grams. Diet. oats. 



November 23: 20 cc 2 per cent caffein (363 mg per kilo) administered by mouth, fol- 

 lowed by 20 cc salt solution; 1.15 p. m., somewhat hypersensitive; 4.30 p. m., tremors 

 fairly marked, no urine passed, about 2 cc of bloody looking urine obtained from blad- 

 der, which contained albumen and a considerable amount of glycogen; rabbit died. 

 Rabbit 419, Belgian hare, male. Weight, 1,600 grams. Diet, oats. 



September 26: 10 a. m., 28 cc 2 per cent caffein (350 mg per kilo) given by mouth; 

 reflexes increased at 4p.m.; 6p.m., reflexes still increased, no other symptoms. 



September 27: 9 a. m., found dead. Autopsy: Lungs, liver, and kidneys congested; 

 other organs normal. 

 Rabbit 420. Belgian hare, male. Weight, 1,250 grams. Diet, oats. 



September 26: 10 a. m., 22 cc 2 per cent caffein (352 mg per kilo) given by mouth; 

 11.35 a. m., convulsions; 12 noon, found dead. Autopsy: Liver showed very extensive 

 coccidiosis; no other lesions. 

 Rabbit 421. Belgian hare, male. Weight, 1,485 grams. Diet, oats. 



September 26: 10 a. m., 26 cc 2 per cent caffein (351 mg per kilo) administered by 

 mouth; 4 p. m., reflexes increased; 6 p. m., reflexes as before, no tetanus observed. 



September 27: 9 a. m., rabbit found dead. Autopsy: Congestion of lungs and kid- 

 neys; liver congested and slightly fatty. 

 Rabbit 424. White, male. Weight, 1,295 grams. Diet, oats. 



September 26: 2 p. m., 19 cc 2 per cent caffein (293 mg per kilo) administered by 

 mouth; 4p.m., reflexes increased, no other symptoms; 6 p. m., no change since 4p.m. 



September 27: 12 noon, convulsions and death. Autopsy: Congestion of the lungs; 

 no other lesions. 

 Rabbit 423. White, male. Weight, 1,205 grams. Diet, oats. 



September 26: 2 p. m., 18 cc 2 per cent caffein administered by mouth; 4 p. m., 

 reflexes increased, no tetanus; 6 p. m., condition unchanged since 4 p. m. 



September 27: 9 a. m., found dead. Autopsy: Lungs liver, and kidneys congested; 

 other organs normal. 

 Rabbit 422. White, male. Weight, 1,440 grams. Diet, oats. 



September 26: 2 p. m., 21 cc 2 per cent caffein (291 mg per kilo) given by mouth; 

 reflexes increased at 4 p. m. 



September 27: 3p.m., alive, no symptoms; 4 p. m., convulsions with recovery, this 

 was soon followed by a violent attack of tetanus, which lasted about one minute and 

 was succeeded by paralysis; rabbit died at 4.30. Autopsy: Liver slightly congested; 

 a small portion of the intestine showed congestion and edema; other organs normal. 



