ACUTE INTOXICATION RABBITS. 29 



SERIES A. 



In this series large doses were administered, approximating 0.3 gram per kilo. 



Rabbit 71. Gray female. Weight, 1,659 grams. 



January 20: 2.20 p. m., 25 cc aqueous solution 2 per cent caffein (300 mg per kilo) 

 were injected into the peritoneal cavity; 3.45 p. m., when doors of cage were opened 

 rabbit had spasm of short duration. 



January 21 : Rabbit found dead. 

 Rabbit 72. Gray and while. Weight, 1,402 grams. 



January 21: 11.17 a. m., 20.2 cc (aqueous solution) of 2 per cent caffein (300 mg 

 per kilo) injected into peritoneal cavity from burette; 11.25 a. m., paralysis; 1 l.::o 

 a. m., rabbit had convulsion when picked up from the floor, followed by several 

 spasms later; 11.35 a. m., typical tetanus; 12.30 noon, found dead. 

 Rabbit 61. Black female. Weight, 2,143 grams. 



January 19: 40 cc 2 per cent caffein, aqueous solution (300 mg per kilo), injected 

 into peritoneal cavity from burette; tetanus when about 30 cc were injected; when 

 removed from holder, repeated and violent convulsions, terminating in death. 



SERIES B. 



The object of these experiments was to determine the minimum lethal dose; 0.2 to 

 0.15 gram of caffein per kilo was injected into the rabbits of this series. 



Rabbit 69. White female. Weight, 1,714 grams. 



January 20: 10.15 a. m., 6 cc 2 per cent caffein, aqueous solution, injected into 

 peritoneal cavity. No symptoms, under observation for 45 minutes, rabbit defecated 

 rather copiously; feces were soft; 11 a. m., 6 cc 2 per cent caffein, aqueous solution, 

 injected into peritoneal cavity, no symptoms, under observation for 40 minutes; 11.40, 

 6 cc 2 per cent caffein injected into peritoneal cavity; 11.45, rabbit is restless, reflexes 

 increased. 

 Rabbit 70. Gray and white female. Weight, 1,487 grams. 



January 20: 1.30 p. m., 15 cc 2 per cent aqueous solution of caffein (0.2 gram per 

 kilo) injected into the peritoneal cavity; 2.20 p. m., no symptoms. 



January 30: About 2 p. m. rabbit died. 



February 1: Autopsy: Cirrhosis of the liver; enteritis of small intestines; stomach 

 and kidneys normal. 

 Rabbit 93. Maltese, male. Weight, 1,197 grams. 



March 2: 11.30 a. m., 12 cc of 2 per cent caffein (200 mg per kilo) injected into 

 peritoneal cavity; 11.35 a. m., while being released from holder, tetanus occurred, 

 repeated attacks later, clonic convulsions with tonic rigidity of posterior extremities 

 during the attacks as well as during intervals, anterior extremities were relaxed during 

 the intervals between the attacks, opisthotonos of cervical region but kyphosis in 

 lumbar region were observed, no salivation nor dilatation of the pupils; 2 p. m., 

 rabbit died. 



Rabbit 92. Yellow female. Weight, 1,388 grams. 



February 25, 4.15 p. m., 14 cc 2 per cent caffein (0.2 gram per kilo) injected into 

 peritoneal cavity; 4.20 p. m., restlessness and increased reflexes, rabbit found stretched 

 out in cage, but raised himself on his legs again; 4.45, general tremor when touched. 



February 26: 9 a. m., rabbit found dead. Autopsy: Hemorrhage into abdominal 

 muscles at site of injection; hemorrhage also in walls of stomach opposite similar 

 spot in abdominal wall. 



Rabbit 309. Belgian hare, female. Weight, 1,500 grams. Diet, oats. 



March 2: 2.05 p. m.,2per cent caffein solution (0.2 gram per kilo) injected into 

 peritoneal cavity; 2.25 p. m., found dead, no urine found in bladder. 

 Rabbit 307. Belgian hare, female. Weight, 1,320 grains. Diet, oats. 



March 2: 12 noon, urine obtained from bladder, clear amber colored, no albumin, 

 no reduction; 12.06 p. m., 10 cc of 2 per cent caffein (0.151 gram per kilo) injected 

 into peritoneal cavity; 1.30 p. m., rabbit placed on floor, runs around but anterior and 

 posterior extremities soon extended, in tonic condition; 2.25 p. m., reflexes increased, 

 paralysis of extremities, dyspnrea; 4.50 p. m., about 100 cc urine collected, no 

 albumin, reduction of Fehling's solution moderate. 



March 3: 9.30 a. m., posterior extremities extended and rigid, anterior extremities 

 paralyzed, respiration less frequent and deeper than normal. Rabbit died at 11.50 

 a. m.; urine collected since 4.50 p. m. previous day gave very heavy reduction of 

 Fehling's solution. Autopsy: Animal in good condition; in the left axillary region 



