30 THE TOXICITY OF CAFFEIN. 



was observed a hemorrhage into the subcutaneous and muscular tissue of that region. 

 The ventral portion of the large colon, in contact with the ventral abdominal wall, 

 showed a hemorrhagic area about one-half inch in length, such as might be produced 

 by a puncture or bruise of the colon through the abdominal wall. A small portion 

 of the small intestine adjacent to the colon, was affected in a similar manner. All 

 internal organs were apparently normal. 

 Rabbti-308. Belgian hare, female. Weight, 1,350 grams. Diet, oats. 



March 2: 11.45 a. m., urine obtained from bladder, no albumen, no reduction; 

 11.50 a. m., 10 cc 2 per cent caffein (0.15 gram per kilo) injected into peritoneal cav- 

 ity; 1.30 p. m., not very active, no abnormal symptoms otherwise; 3. 30 p. m., rabbit 

 looked depressed, made veiy little attempt to move about, remained in one position 

 most of the time when placed on floor; 4.30 p. m., 180 cc urine collected, no albumen, 

 reduction of Fehling's solution moderate. 



March 3: 9.30 a. m., rabbit looks normal, is able to walk but is easily fatigued when 

 made to walk about or when placed on his side, followed by paralysis of anterior 

 extremities, posterior extremities apparently normal, about 90 cc of urine collected 

 at noon was free from albumen, did not reduce Fehling's solution. 



March 4: 11 a. m., lying on his side in cage, anterior extremities limp, posterior 

 extremities extended and rigid, is in dying condition. 



March 5: 9 a. m., found dead. Autopsy: Liver engorged; spleen congested, but 

 not enlarged; kidneys, some congestion in cortex; stomach filled, mucosa thickened 

 and easily pulled off; petechial hemorrhages on serosa of colon. 



SERIES C. 



The experiments of this series were made to determine the minimum toxic dose. 

 Rabbit 295. Belgian hare, female. Weight, 1,205 grams. Diet, carrots. 



March 1: 10.40 a. m., 6 cc 2 per cent caffein (0.1 gram per kilo) injected into peri- 

 toneal cavity; about 2 cc of urine obtained before injecting caffein; 2 p. m, 100 cc 

 urine, bloody in appearance, collected, a moderate quantity of albumen present, no 

 reduction; 3.40 p. m., no symptoms. 



March 4: 2 p. m., rabbit looks well. 

 Rabbit 293. Belgian hare, female. Weight, 1,605 grams. Diet, carrots. 



March 1: Urine from bladder clear, alkaline; 11.55 a. m., 8 cc 2 per cent caffein 

 (0.1 gram per kilo) injected into peritoneal cavity; 3 p. m., 90 cc urine normal in color 

 collected, no albumen, no reduction; 3.40 p. m., no symptoms. 



March 4: 1.15 p. m., rabbit looks normal. 

 Rabbit 292. Belgian hare, male. Weight, 1,595 grams Diet, carrots. 



March 1: 10.10 a. m., 8 cc 2 per cent caffein (0.1 gram per kilo) solution injected 

 into peritoneal cavity; 10.40 a. m., rabbit urinated, reflexes increased, but no other 

 symptoms; 10.50 a. m., no urine obtained from bladder; 2 p. m., 105 cc of clear pale 

 urine collected; no albumen, no reduction; 3.40 p. m., no symptoms. 



March 4: 2 p. m., rabbit looks well, urine collected, did not contain sugar. 

 Rabbit 298. Belgian hare, female. Weight, 1,205 grams. Diet, carrots. 



March 1: 4.06 p. m., 7.5 cc 2 per cent caffein solution (0.125 gram per kilo) injected 

 into peritoneal cavity, urine obtained from bladder immediately after injection, no 

 albumen, no reduction; 5.30 p. m., reflexes increased, rabbit was able to run around, 

 but became paralyzed soon; 5.40 p. m., rabbit is again able to run around. 



March 3: 10 a. m., anterior extremities paralyzed, is able to use posterior extremi- 

 ties. 



March 4: 1 p. m., rabbit looks normal. 

 Rabbit 223. Belgian hare, male. Weight, 1,165 grams. Diet, carrots. 



March 1: 3.50 p. m., urine obtained from bladder clear, amber colored, no albumen, 

 no sugar; 3.52 p. m., 7.5 cc 2 per cent caffein (125 mg per kilo) injected into peri- 

 toneal cavity; 5.40 p. m., rabbit makes little attempt to run when put on the floor, 

 weakness of extremities marked. 



March 4: 1.15 p. m., rabbit normal. 



SERIES D. 



The object of the experiments of this series was to study the effect of age on the 

 resistance to caffein. Half-grown rabbits were, therefore, used in the following experi- 

 ments. 

 Rabbit 310. Belgian hare, female. Weight, 880 grams. Diet, oats. 



March 2: 3.25 p. m., 9 cc 2 per cent caffein (0.2 gram per kilo) injected into peri- 

 toneal cavity. 



