34 THE TOXICITY OF CAFFEIN. 



Rabbit 285, yellow and white, female. Weight, 1,385 grams. 



December 14: 10.15 a. m., 14 cc 2 per cent caffein injected into the gluteal muscles 

 (0.2 gram per kilo), general tremors, but no convulsions observed. Rabbit survived. 



December 17: Rabbit still alive. 

 Rabbit 287. Belgian hare, female. Weight, 1,140 grams. 



December 15: 2.15p.m., lice of 2percent caffein injected into the gluteal muscles; 

 2.30 p. m., tonic contractions of posterior limbs. Paralysis and death at 2.40 p.m. 



SERIES B. 



In series B the caffein was injected into the lumbar muscles. 



Rabbit 307. Belgian hare, female. Weight, 1,175 grams. 



February 16: 11.05 a. m., 8 cc 2 per cent caffein injected (0.136 gram per kilo) into 

 the lumbar muscles; under observation until 4 p. m., no symptoms; 4p.m., allowed 

 to walk on the floor ; after walking a short distance loss of coordination and paralysis 

 of posterior extremities; 5.20 p. m., found dead. 

 Rabbit 306. Belgian hare, female. Weight, 1,860 grams. 



February 16: 11 a. m., 12.5 cc 2 per cent caffein injected into the lumbar muscles; 

 12 noon, no symptoms; 2 p. m., walked about 10 feet, exhaustion and paralysis; 



3 p. m. found dead. 



Rabbit 181. Belgian hare. Weight, 1,230 grams. (Was experimented on some 



time previously.) 



February 16: 10.55 a. m., 8 cc 2 per cent caffein injected into the lumbar muscles; 

 (0.130 gram per kilo); 12 noon, no symptoms; 2 p. m., no symptoms; 3 p. m., put on 

 the floor, walked about 10 feet ana was exhausted, posterior extremities paralyzed; 



4 p. m., found dead. 



SERIES C. 



In the fall of the same year additional experiments were carried out with doses 

 ranging from 100 to 200 milligrams of caffein per kilo, which were injected into the 

 lumbar muscles. The results are given in the following abbreviated protocols: 



Rabbit 425. Belgian hare. Weight 1,520 grams. 



September 27: 10.30 a. m., 7.5 cc 2 per cent caffein injected into the lumbar mus- 

 cles; 2 p. m., reflexes increased. 



September 28: Rabbit normal. 



October 5: Weight, 1,620 grams; 2.50 p. m., 10 cc 2 per cent caffein injected into 

 lumbar muscles; 3.05 p. m., reflexes increased. 



October 13: Weight, 1,520 grams; 10.30 a. m., 10 cc 2 per cent caffein (131 mg per 

 kilo) injected; 11 a. m., no symptoms; 11.30 a. m., reflexes much increased. 



October 14: Alive, no symptoms. 

 Rabbit 426. Belgian hare, female. Weight, 1,425 grams. 



September 27 : 7 cc 2 per cent caffein injected into the lumbar muscles at 10.30 a. m. ; 

 2 p. m., reflexes increased. 



September 28: Rabbit normal. 



October 5: Weight, 1,425 grams; 2.55 p. m., 9 cc 2 per cent caffein injected into lum- 

 bar muscles; 3.05 p. m., reflexes increased. 



October 13: Weight, 1,405 grams; 10.30 a. m., 10 cc 2 per cent caffein (142 mg per 

 kilo) injected; 11 a. m., no symptoms; 11.30 a. m., reflexes increased. 



October 14: Rabbit alive, no symptoms. 

 Rabbit 427. Belgian hare, female. Weight, 1,780 grams. 



September 27: 9 cc 2 per cent caffein injected into the lumbar muscles; 2 p. m., 

 reflexes increased. 



September 28: Rabbit normal. 



October 5: Weight, 1, 850 grams; 3 p. m., 11.5 cc 2 per cent caffein injected into 

 lumbar muscles; 3.10 p. m., reflexes increased. 



October 13: Weight, 1,830 grams; 10.40 a. m., 14 cc 2 per cent caffein (153 mg per 

 kilo) injected into lumbar muscles; 11 a. m., no symptoms; 11.30 a. m., reflexes 

 increased. 



October 14: Rabbit alive, no symptoms. 

 Rabbit 453. Belgian hare, male. Weight, 1,160 grams. 



October 12: 3.45 p. m., 11.5 cc 2 per cent caffein in aqueous solution injected into 

 lumbar muscles; 4.15 p. m., reflexes increased; 4.30 p. m., paralyzed. 



