ACUTE INTOXICATION BABBITS. 35 



October 13: 9 a. m., found dead. Autopsy: Gastric mucosa hemorrhagic; liver 

 darkened; other organs normal. 

 Rabbit 455. Belgian, hare, gray, female. Weight, 1,185 grams. 



October 12: 3.30 p. m., 11.5 cc 2 per cent caffein injected into the lumbar muscles; 

 4 p. m., reflexes increased. 



October 13: Rabbit weighed 1,070 grams, no symptom of caffein poisoning, reflexes 

 normal; 10.30 a. m., 10 cc 2 per cent caffein injected into the lumbar muscles; 11.30 

 a. m., jumped off the table, nad attack of convulsions and died. Autopsy: Findings 

 same as in No. 453. 

 Rabbit 428. Belgian hare, gray, male. Weight, 1,650 grams. 



October 5: 4 p. m., 14.8 cc 2 per cent caffein (0.18 gram per kilo) injected into the 

 lumbar muscles. 



October 6: Found dead. 

 Rabbit 429. Belgian hare, male. Weight, 1,340 grams. 



October 5: 4 p. m., 13.5 cc 2 per cent caffein (0.2 gram per kilo) injected into lum- 

 bar muscles. 



October 8: Rabbit found dead. 



SERIES D. 



Further experiments making injections into both the lumbar and the gluteal muscles, 

 were made in this series. 



Rabbit 577. Gray male. Weight, 1,380 grams. 



February 14: 3 p. m. 14 cc 2 per cent caffein injected into the gluteal muscles of 

 the right side; 3.10 p. m., restless, jumped off the table and walked about, reflexes 

 increased; 3.45 p. m., passed 30 cc clear, straw-colored urine; 4.45 p. m., allowed to 

 walk about, ran across the room, about 20 feet, looked tired, stretched himself out on 

 the floor, then raised himself and walked about showing no disturbance of gait. 



February 15: 9 a. m., found dead. 

 Rabbit 578. Gray, female. Weight, 1,670 grams. 



February 14: 3.05 p. m., 18 cc 2 per cent caffein solution injected into the gluteal 

 muscles of the right side; 3.15 reflexes increased, but not restless; 5 p. m., allowed to 

 walk about, no symptoms observed. 



February 15: Found dead. 

 Rabbit 579. White and gray, male. Weight, 1,490 grams. 



February 14: 3.15 p>. m., 15 cc 2 per cent caffein solution injected into the gluteal 

 muscles of the ri^ht side; put in cage; 3.30 p. m., reflexes increased; 5 p. m., taken 

 out of cage and allowed to walk across the room, no special symptoms noticed. 



February 23: Still alive. 

 Rabbit 580. Gray male. Weight, 1,510 grams. 



February 14: 3.35 p. m., 15 cc 2 per cent caffein solution injected into lumbar 

 muscles. 



February 23: Still alive, in good condition. 

 Rabbit 581. Gray female. Weight, 1 ,680 grams. 



February 14: 3.45 p. m., 17 cc 2 per cent caffein solution injected into the lumbar 

 muscles of the right side; 4 p. m., reflexes increased; 4.15 p. m., jumped off the table 

 and had wild convulsions, became very restless, walked about the laboratory; 4.25 

 p. m., had convulsions occasionally; 4.30 p. m., extremities extended and quite rigid; 

 4.35 p. m., convulsions and death. 

 Rabbit 582. Gray male. Weight, 1,870 grams. 



February 14: 4.15 p. m., 18 cc 2 per cent caffein solution injected into the lumbar 

 muscles of right side; 5 p. m., reflexes increased; walked about in the room, then 

 rested; 5.15 p. m., had short spasm when handled. 



February 23: Alive; good condition. 



The data presented in these experiments show that the toxicity of caffein when 

 injected into the muscles of the lumbar regions is the same as when injected into the 

 gluteal muscles. The rabbits of series A received approximately 0.2 gram caffein per 

 kilo and two died as a result of this treatment. The other two survived but symptoms 

 of caffein intoxication were observed. 



In series B smaller doses proved fatal, from which it would appear that caffein is 

 more toxic when injected into the lumbar muscles. Further observations, however, 

 failed to corroborate the results obtained in this series. Thus, in series C, 130 to 150 



