CHRONIC INTOXICATION RABBITS. 



65 



Rabbit ItS. White, female. Diet, oats. 

 Received caffein subcutaneously as follows: 



Rabbit died 30 minutes after last injection of caffein. Autopsy: Stomach exhibited 

 marked inflammation of mucosa. Slight enteritis. Liver and kidneys were deeply 

 congested and dark colored. 



Rabbit No. 101, white male. Diet, oats. 

 Received caffein subcutaneously as follows: 



April 5: Found dead. Autopsy: Marked inflammation of gastric mucosa. Con- 

 siderable enteritis affecting the whole extent of the intestines; liver congested and 

 friable; kidneys deeply congested in cortical and medullary portions; spleen con- 

 gested, but of normal size; lungs and heart normal. 



Four days, 0.1 per kilo; 10 doses in 14 days. 

 Rabbit 201. Diet of carrots begun October 1; October 19, oats. 

 . Subcutaneous injections as follows: 



Under observation six hours October 20; no symptoms. 



October 23: Died; was much emaciated but did not show any symptoms; emaciation 

 set in when caffein was withdrawn; urine never contained sugar or albumen; symp- 

 toms observed after second dose only. 



SERIES B. 



The question whether caffein is cumulative in the rabbit, suggested in the preceding 

 experiments, was the subject of further investigation in Series B. Caffein was given 

 by mouth or subcutaneously. Carrots formed the exclusive diet, a measured amount 

 being given. The rabbits were kept under observation for two weeks, except 

 Nos. 370 and 373, records of which were made only for four days before the administra- 

 tion of caffein was begun. Caffein was given by mouth in experiments of Groups I 

 and III. Rabbits 292, 293, and 295 received daily 20 cc water by mouth for four days 

 previous to the administration of caffein, while in the rabbits of Group II the caffein 

 treatment was preceded by the injection of 0.8 per cent salt solution subcutaneously. 

 The object in both cases was to ascertain whether or not the method of the adminis- 

 tration of caffein has any influence on the animal, but observation made from day to 



