CHRONIC INTOXICATION DOGS. 77 



grams in 18 days. Autopsy: Post-mortem examination showed marked enteritis 

 with hemorrhaeic spots on the mucosa; liver and kidneys congested and dark colored; 

 lungs congested; thyroid gland was greatly enlarged and congested. 



Doa ZS: 



April 30: Weight, 6.8 kilos; the diet consisted of 250 grams rice, 250 grams sugar, 

 100 grams cracker meal, and 100 grams of meat. On May 3 his weight was 7 kilos. 

 He received 1 gram of caffein by mouth at 12 noon. At 2 p. m. he vomited and 

 tremors were observed. The next day, .May 4, tremors were still present though less 



Counced. Examination of the urine for sugar and albumin was negative; on 

 4, 1 gram caffein was given again and repeated on May 5. On this date his general 

 condition was not good dog had no appetite and refused, to take caffein. As the dog 

 lost 10 per cent of his weight he wasput on a meat diet exclusively and the dose of 

 caffein was reduced to 0.5 gram. He became sick after the second dose, and the 

 administration of caffein was therefore discontinued. It was resumed after five days 

 and the caffein was administered in increasing amounts, i. e., on May 18, 0.5 gram; 

 May 19, 0.5; May 20, 1; May 21, 1 gram in two doses of 0.5 each, given at intervals of 

 one hour; May 22, 1 gram. Dog became irritable, but no other symptoms were 

 observed. The administration of caffein was omitted the next day. On the follow- 

 ing day when the same dose of caffein was given there was again marked irritability 

 and tremors. The experiment was therefore discontinued. 

 Dog 22. Male bulldog. 



June 24: Dog weighed 13.7 kilos. Diet consisted of meat exclusively; 1 gram 

 caffein was given by mouth; diarrhea developed; no caffein was given for three 

 days. 



June 28: Dog weighed 13.6 kilos, 1.5 grams caffein given at 10 a. m. 



June 30: 1.75 grams caffein administered. 



July 2: Dog weighed 13.5 kilos; 2 grams caffein or 0.15 gram per kilo, caused well- 

 marked thirst, but did not produce any other symptoms. 

 Dog 20. Female: 



May 12: Weight, 7.7 kilos. Fed liberal carbohydrate diet, consisting of rice, 100 

 grams; sugar 100 grams; meat and cracker meal, a sufficient quantity to flavor the 

 food. 



May 14: Weight, 7.7 kilos. Examination of urine for albumin and sugar gave 

 negative results. Urine was acid to litmus. 



May 17: Weight, 7.4 kilos. Three hours after it was fed the dog received 0.5 

 gram caffein by mouth. The test of the urine the next day for sugar was negative, 

 but a trace of albumin was present. It will be noticed that the doses were increased 

 gradually and that symptoms were observed only after the fourth dose of 0.1 gram 

 per kilo. Later meat was substituted for the carbohydrate diet and the administra- 

 tion of caffein was stopped for four days. At the end of this period 100 mg caffein 

 B3r kilo was fed daily for five days, and the dose was then very gradually increased, 

 iarrhea occurred twice, but no other symptoms, the secona attack having lasted 

 a few days. The following is a complete record of the experiment: 



May 19: 0.5 gram caffein 11.45 a. m. 



May 20: 0.75 gram caffein 12.45 a. m. 



May 21: 0.75 gram caffein 12 noon; no sugar, no albumin in urine. 



May 22: 0.75 gram caffein; urine, same condition found; no symptoms. 



May 23: Weight, 7.5 kilos; no caffein. 



May 24: 0.75 gram caffein ; tremors very marked. 



May 25: No caffein. 



May 26: 0.75 gram caffein. 



May 27: 0.75 gram caffein. 



May 28: 0.75 gram caffein. 



May 29: 1 gram caffein in two doses of 0.75 and 0.25 gram. 



May 30: No caffein. 



May 31: No caffein; meat diet exclusively. 



June 1: No caffein; meat diet exclusively. 



June 2: No caffein; no sugar, no albumin in urine. 



June 3: Weight, 7.6 kilos; 0.75 gram caffein; no sugar in urine. 



June 4: Weight, 7.3 kilos; 0.75 gram caffein; no sugar in urine. 



June 5: Weight, 7.5 kilos; 0.8 gram caffein; drank 500 cc water; ate 200 grams meat. 



June 6: Weight, 7.4 kilos; 0.8 gram caffein; 500 cc urine; drank 500 cc water; ate 

 200 grams meat; no symptoms. 



June 7: Weight, 7.7 kilos; 0.8 gram caffein 10 a. m.; 400 cc urine, 500 cc water, 200 

 grams meat. 



