CHBONIC INTOXICATION DOGS. 83 



p. m., looked and behaved as if very sick, no other symptoms; 3.45 p. m., in attempt in;; 

 to get out of box fell over on back, had convulsions, wnined, dyspnoea, died within :'.o 

 seconds. 



Autopsy: Stomach exhibited mild inflammation; small intestine inflamed and hem - 

 orrhagic areas on mucosa; liver engorged and friable; spleen normal; kidneys slightly 

 congested; other organs appeared normal. The total amount of caffein fed to Dog 29 

 was 12.135 grams, which was given in 18 days. The average daily amount per kilo 

 was therefore 67.68 mg. 

 Dog S8. Male fox terrier. 



Low nitrogen diet was begun about four weeks before the feeding of caffein; 50 mg 

 of caffein was then fed for seven consecutive days. Partial loss of appetite was 

 observed after the first dose. As the experiment progressed the desire for food steadily 

 diminished, and the feces became fetid. Symptoms of intoxication manifested them- 

 selves early in the experiment, and vomiting occurred after the fourth dose. The 

 dog was then put on a diet exclusively of meat. After an intermission of 10 days 109 

 mg caffein per kilo were given. Since there were no symptoms, the following day the 

 amount was increased to 125 mg per kilo. This dose proved fatal within 16 to 20 hours. 



This dog was stout and strong, weight 12.25 kilos, received daily 0.269 gram nitrogen 

 per kilo (88.269 calories per kilo). 



Novembers: Weight, 11.75 kilos. 



November 10: Weight, 11.95 kilos. 



November 20: Weight, 11.20 kilos. All through this period had been kept in a cold, 

 poorly ventilated room, put in a warm room, with bedding and good ventilation. 



November 29: Weight, 11.95 kilos. 



December 1: Put in a cage; weight, 11.95 kilos. 



December 6: Weight, 11.95 kilos; 11.45 a. m., received 0.050 gram caffein per kilo; 

 theh received 30 cc 2 per cent caffein (0.6 gram) in practically one subcutaneous injec- 

 tion; 4.30 p. m., ate only part of food. 



December 7: 10.25 a. m., received 30 cc 2 per cent caffein by subcutaneous injec- 

 tion (0.6 gram, or 50 mg, per kilo); 1.45 p. m., seemed sensitive to touch, no desire for 

 food, depressed in spirit. 



December 8: 11.40 a. m., received 30 cc 2 per cent caffein by subcutaneous injec- 

 tion (50 mg per kilo); 1 p. m., depressed in spirit, hind legs seemed somewhat stiff, 

 no desire for regular food, site of injection inflamed. 



December 9: 10.50 a. m., received 30 cc 2 per cent caffein by subcutaneous injec- 

 tion (50 mg per kilo); 2.30 p. m., had vomited, no desire for regular food. 



December 10: Inflammation of site of injection, and swelling very pronounced; 

 2p.m., received 0.5975 gram caffein, or 50 mg per kilo, with 30 grams of meat, refused 

 regular food. 



December 11, 12: Received 0.5975 gram caffein by mouth, no symptoms except 

 refusal of regular food, feces fetid. 



December 13-22: Put on meat diet exclusively, high temperature, no caffein, 

 weight 10 kilos, appetite good, feces fetid. 



December 22: 12m., weight 11 kilos, received 1.2 grams caffein by mouth (0.109 

 gram per kilo); 4 p. m., no symptoms. 



December 23: 11.30 a. m., received 1.375 grams caffein (0.125 gram per kilo) had 

 vomited food of the day before, but could notice no caffein or capsules ill vomit; 4.30 

 p. m., no symptoms, seemed in good spirits, appetite good, had no meat to feed with, so 

 was given low nitrogen feed, of which he ate about one-fourth. 



December 24: 9 a. m., found dead, stiff, and cold. The most striking effect of caffein 

 in this dog is the increased intestinal putrefaction. The feces were still fetid 10 days 

 after the administration of caffein was stopped. 



Autopsy, dog 28: Stomach partially filled with an undigested food mass; mucosa 

 showed severe inflammation; small intestines presented a hemorrhagic enteritis along 

 whole extent; large intestine also exhibited mild inflammation; liver was engorged; 

 spleen appeared normal; kidneys slightly congested in cortical portion; other organs 

 appeared normal. 



Dog 24. White and tan male: Was put on low protein diet six weeks before experi- 

 ments with caffein were begun. The initial dose of 50 mg per kilo was then admin- 

 istered on eiht consecutive days. The only symptoms observed during this period 

 of caffein administration were those of intestinal putrefaction. Fetid feces were 

 noticed already after the first dose of caffein was injected. When the second dose 

 of 75 mg of caffein was repeated, mild symptoms appeared, but none have been 

 observed even with increased amounts or caffein. 



One-third nitrogen diet. Received daily 0.269 gram nitrogen per kilo (88.269 

 calories per kilo). 



