64 BACTERIOLOGY. 



with puerperal fever was injected with 8 cc., on the follow- 

 ing day with 16 cc., and on the third day with 25 cc. On the 

 fourth day the temperature had fallen, and the patient recovered. 

 Favourable results are said to have followed the use of the serum 

 in 46 cases of erysipelas. 



Bokenham, working independently, cultivated the streptococcus 

 in a mixture of broth and serum. Horses and asses were inocu- 

 lated, and a considerable degree of immunity established. The 

 serum of an inoculated ass possessed antitoxic power. 



Ruffer and Bullock succeeded in immunising four horses against 

 the toxin of Streptococcus pyogenes ; two had been previously immu- 

 nised against the toxin of the diphtheria bacillus. The streptococcus 

 was cultivated by Marmorek's methods in a mixture of two parts 

 of blood-serum and one part of peptonised broth, and the virulence 

 of cultures maintained by inoculation of rabbits. On testing the 

 immunising power of the antitoxic serum on rabbits, the effect 

 appeared to be slight in comparison with the antitoxins of the 

 bacilli of diphtheria and tetanus. In treating cases of septic infection 

 in the human subject, it has been recommended to commence with 

 two injections of 10 cc., and it is said that no unfavourable results 

 have been met with which could be attributed to the effect of the 

 serum. 



ANTITOXIN OF TYPHOID FEVER AND OTHER DISEASES. 



An antitoxic serum has been obtained by Chantemesse for use in 

 cases of typhoid fever, and it is probable that attempts will be made 

 to extend the principle of the antitoxic treatment to other infective 

 diseases. 



