NUTKIKNT MEDIA AND METHODS OF CULTIVATION. 



Ill 



completely removed from the gelatine. It is, however, not easy to 

 lit* successful at first, but with practice this can be accomplished 

 with rapidity and precision. A preparation is then made by rubbing 



FIG. 40. PASTEUR'S LARGE INCUBATOR. 



the extremity of the needle in a droplet of water on a slide, covering 

 with a cover-gla. and examining in the fresh Mate, in- l>y spreading 

 nut th- droplet on a co\vr-gl:t>>. drying, );issing three times through 

 the riame. and staining witli a drop of fuchsine or gentian violet. 

 Inoculation.- should lie made in te>t-tube> of nutrient gelatine 



