MT1THATION, I'Y^MIA, SEPTICAEMIA, ERYSIPELAS. 187 



with Streptococcus pyogenes. ha\e heen found in cattle plague, font 

 ami mouth di>rase. wrangles, contagious inaininitis in cows, and 

 progressive tissue necrosis in mice, and they will be referred to fully 

 in subsequent chapters. 



EXAMINATION- AND CULTIVATION OF STREPTOCOCCI. 

 < '<>ver-glass preparations can be stained with the watery solutions 

 of the aniline dyes. In some cases very beautiful preparations can 

 he obtained by using Neelsen's solution, and removing excess of 



FIG. 89. STREPTOCOCCUS PYOGENES HOMINIS. 



gelatine. 



Pure-cultures on nutrient 



a, Sub-culture from agar. 

 r, Sub-culture from milk. 



6, Sub-culture from broth. 

 d, Sub-culture from milk. 



>tain by rinsing in alcohol. To examine pus, milk, or broth, take 

 an ordinary platinum needle bent at the extremity into a booklet. 

 Dip it into the liquid to be examined, and spread it on a cover - 

 irl;i into as thin a film a> po>>ihle ; the preparation is treated 

 in the ordinary way, that is to say. the film is allowed to dry. 

 and the cover is taken up with forcep>. and paed three time> 

 through the flame with its prepared side upperino-t. 



ti i'n in'. < M<>ili<J n-'ith Eosin. -In thi> way th- streptococci are 

 >tained blue, and stand out in mark' d contra>t to the rest of the 

 preparation. 1 >e fre-ldy prepared solution. Float the cover-glasses 



