218 



INFECTIVE DISEASES. 



Involution forms are freely developed in old cultures, and in 

 cultures made in unsuitable media. The bacilli possess numerous 



flagella, and their 



power of movement at 



^p once disting uishes them 



. * from anthrax bacilli. 



J 4? They can be cultivated 



\ , in the ordinary media 



o _ O o in the absence of oxy- 



gen, but more readily 

 j with the addition of 

 grape-sugar or glyce- 

 rine. Radiating fila- 



01 ments grow out from 



. ^ the more or less spher- 

 ^ ical colonies directly 



FIG. 101. BACILLI OP QUARTER-EVIL x 1000. From 

 an agar culture (FRANKEL and PFEIFFER). 



liquefaction commences. In the depth of 

 nutrient gelatine the growth occurs in two 

 or three days at 20 to 25 0. towards the 

 lower part of the track of the inoculating 

 needle. The gelatine slowly liquefies, and 

 there is considerable formation of gas with 

 the development of a peculiar odour. Spore- 

 formation occurs freely in cultures, but not 

 in the blood of infected animals until after 

 death. 



Guinea-pigs inoculated with a pure- 

 culture, or with spore-bearing threads, die 

 in twenty-four to thirty-six hours. An em- 

 physematous infiltration with sanguineous 

 serum is produced at the seat of inoculation, 

 and the surrounding muscles are of a dark 

 colour. The internal organs are more or less FlG 102 P UBE _CULTUHK OF 

 congested. The bacilli are found in the BACILLI OF QUARTER-EVIL 



local exudation and in the surrounding IN GI^PE-SUGAR GELA- 

 TINE (FRANKEL and 

 tissue, and some hours after death in PFEIFFER). 



