ACTINOMYCOSIS. 443 



TRANSMISSION OF ACTINOMYCOSIS FROM MAN TO THE LOWER ANIMALS. 



Inoculation of cultures has already been referred to. The 

 author successfully inoculated a calf with material direct fn.ni 

 a living patient. 



A calf which had been inoculated in the peritoneal cavity, a ml 

 killed seventy days afterwards, presented the following lesions. The 

 peritoneum of the rumen, in the vicinity of the seat of iim< -ul .,- 

 was studded with hundreds of growths, varying in size from a millet - 

 seed to a pea. The large growths were composed of several small 

 ones collected together. On stripping off the peritoneum, and holding 

 it between the light and the eye, the fungus could be seen with 

 the naked eye in each individual growth. By incising a growth. 

 and examining a scraping under the microscope, the diarart-ri.>tir 

 clubs, and the filaments also, were found to be present. By staining 

 cover -glass preparations with the method of Gram and orange-rubin, 

 the appearances were very striking. The clubs were conspicuoi 

 account of their size, and brilliantly stained. In many, the proto- 

 plasm of the thread was demonstrated in the interior of the club. 

 In sections of the peritoneal nodules stained by Gram's methyl 

 the mycelium was found to be present, and the clubs in part 

 took the stain. With Plant's method the clubs were most dearly 

 demonstrated. 



l>rael and Johne failed to infect a calf by intravenous injec- 

 tion, and Ponfick failed to infect dogs, but Israel succeeded with 

 a rabbit, Israel obtained a small piece of actinomycotic granula- 

 tion tissue from a peri-pleural abscess in a patient with primary 

 disease of the lung, and introduced it into the peritoneal Of 

 The rabbit showed no sign of illness, and was killed ab".,t t.-n 

 weeks afterwards. On examination numbers of tumours were 

 found in the abdominal cavity, varying in si/.e from a hemp-seed 

 to a cherry. Larger ones had a somewhat nodular Mirtac- with 

 yellowish points; others were found on the abdominal wall .n 

 the right side. There was a small growth over the psoas muscle, 

 and one large one attached to an adhesion of th- >1<>>. 

 growths were on the peritoneum, or attached by longer or * 

 adhesions. Some of the larger turnout >h..wed on section a h.ll. 

 space in the centre, which was filial with a pulp, ti 

 fatty degeneration of the traii>plant.-d time, whidi ** 

 differentiated in colour and consistency from the new gr 

 latter consisted of -ranulation fcURM with ahundant 

 fat granules, blood pigment, acicular tat eryttek, and acti 



