ACTINOMYCOSIS. \ \' t 



deposit resembling a fibroma. The cow was sla tight nvd l:;;j , 

 after inoculation. Typical actinomycosis had heen produced. 



A colt three and a half years old, was inoculated in the jaw 

 and in the forehead after trephining, and also in the trachea. Tin- 

 animal died without any result. 



Ponfick also conducted a series of experiment- which amply 

 confirmed the results which had been obtained by John.-. 



Feeding Experiments. Repeated experiments, made with mnmcc 

 of the growth chopped up, or with isolated grains of the fungus, : 

 negative results. 



Inoculation Experiments. The growth was inoculated in various 

 regions of the body. Small particles of the growth from quite fresh 

 tumours were introduced into the anterior chamber of the eye in 

 rabbits, with negative results. Rabbits were inoculated in the 

 peritoneal cavity from an animal recently slaughtered, but they 

 died of peritonitis. In dogs also the results were negative. 



Seven calves were operated on. In five the abdomen was opened 

 with antiseptic precautions, and in two cases the grout h 

 introduced by injection. In the latter cases the pieces of tumour 

 were suspended in salt solution, but the animals died from peritonitis 

 a few days after the injection. The same result occurred in two out 

 of the five cases in which the abdomen was opened. The three 

 remaining cases gave the following results : 



1. Pieces of tissue, about 1'5 cm. long, were taken from the 

 lower jaw of a recently killed ox. Twelve of these pieces 

 introduced into the peritoneal cavity ; death occurred after 

 days, from exhaustion and recent lobular pneumonia. At the p 

 mortem examination several patches of peritonitis w-n- found, with 

 encystment of the remains of fragments of the inorulati 

 but there was an independent development of several nodules in tin- 

 neighbourhood of the stomach and urinary Madder. Examination 

 of these new formations showed, even to the nal, he\ 



contained yellowish grains, which, on further examination, pi 

 to be the fungi. 



2. Ten pieces of tumour from the jaw of a row pert 

 as before; the calf died suddenly sixty day- atWwanK d 

 injection of fresh pieces of actinomyco-i- into the ju-ul ..r 

 the jJost mortem it was found that various adheoou had occurred, 

 as the result of peritonitis; and in tin* tal>' nn'ml'i 

 sixty-three nodules, varying in si/,-. MkraMOpfcftl 

 showed That all these nodules consisted of typical acti,, 

 formation. 



