446 INFECTIVE DISEASES. 



3. In this case twelve pieces of growth were introduced into 



the abdomen of a calf eight weeks old. Seven days afterwards fresh 

 pieces were introduced under the skin, in the region of the left lower 

 jaw. A swelling occurred, which was opened. Pus escaped, 

 together with the material which had been used for inoculation, in 

 a state of decomposition. Pieces of tumour were inoculated sub- 

 cutaneously in the neighbourhood of the right side of the neck, 

 ninety-nine days after the first experiment. The animal was bled to 

 death seven months (210 days) after the first experiment. At the 

 post mortem, peritonitis and adhesions were found, with twenty-one 

 large and several small nodules in the mesentery, in the false mem- 

 branes between the viscera, and on and in the serous linings of most 

 of the abdominal organs. There were also several large and many 

 small tumours in the subcutaneous and intermuscular tissue, in the 

 region of the lower jaw and neck on the right side, and numerous 

 large and small nodules in both lungs, some undergoing softening 

 in the centre. 



Isolated fungi were inoculated in dogs, with negative results, 

 nothing remaining after 45 to 80 days, except a thick emulsion. 



Pieces of tumour were introduced in the submucous and sub- 

 cutaneous tissue of dogs, but no change occurred after 600 days. 



In a calf, inoculation under the gum of the upper jaw showed 

 w T hat was possibly only the remains of the growth which had been 

 introduced. At any rate, the experiment was doubtful ; but in 

 a second calf there were numbers of nodules developed around 

 the points of inoculation in the subcutaneous and muscular tissue 

 in the neighbourhood of the lower jaw, and in the region of the 

 neck. The results were observed in these regions in 210 and 110 

 days respectively. 



Inoculation of rabbits and dogs, with isolated fungi, produced 

 no results after 156, 165, and 170 days. Experiments on dogs, 

 with grains from a human source, were also unsuccessful. They 

 were examined after 470 days, and there was no sign of any 

 result. 



After intravenous injection, positive results were discovered m 

 the only calf which survived this operation, on bleeding the animal 

 to death 110 days afterwards. Numerous nodules (27) were dis- 

 covered in the parenchyma of both lungs, without suppuration. 



Two dogs were injected in the jugular vein with isolated fungi 

 mixed with 60 grammes of salt solution. Examined after 45 and 

 80 days respectively, the lungs and all the other organs were found 

 to be free from growths. 



