<618 APPENDICES. 



17. Gibbes' solution, for double staining. 

 Take of 



Rosaniline hyclrochlorate ..... 2 



Methylene-blue . . . . . . 1 



Triturate in a glass mortar. 



Dissolve aniline oil . . . . . . 3 



In rectified spirit . . . . . .15 



and add slowly to the above. 



Lastly, slowly add distilled water . . .15 

 Keep in stoppered bottle. 



18. Glycerine, pure. 



19. Haematoxylin solution. 



Hsematoxylin . . . . . . . 2 



Alcohol . 100 



Distilled water 100 



Glycerine ....... 100 ' 



Alum 2 



20. Iodine solution. 



Iodine, pure ....... 1 



Iodide of potassium . . . . . 2 



Distilled water ...... 50 



21. Iodine solution (Gram). 



Iodine . . . . . . . . 1 



Iodide of potassium 2 



Distilled water 300 



22. Lithium- carmine solution (Orth). 



Saturated solution of carbonate of lithium . 100 

 Carmine . . . . . . . . 2-5 



23. Magenta solution (Gibbes). 



Magenta . . . . . . . . 2 



Aniline oil ....... 3 



Alcohol (sp. gr. -830) . . . 20 



Distilled water 20 



24. Methylene-blue. 



(a) Concentrated alcoholic solution. 



(b) Aqueous solution. 



Methylene-blue ... . . . . 2 



Alcohol . . . . . . . .15 



Water 85 



