TABLE OF CONTENTS XI 



PAGE 



The composition and preparation of media 67 



Liquid media 68 



Solid media 71 



Liquefitable solid media 73 



Synthetic media 75 



CHAPTER VII 



STERILIZATION 



Introduction 77 



Sterilization by physical agents 78 



Sunlight 78 



Cold . 78 



Dry heat 79 



Direct flame 79 



High dry heat . . 79 



Moist heat 80 



Streaming steam 80 



Steam under pressure 82 



Disinfection 84 



CHAPTER VIII 



CULTURE METHODS AND APPARATUS 



Introduction 85 



Methods of isolation 86 



Fractional methods 86 



Physiological methods ........ 86 



Dilution methods ......... 87 



The use of liquefiable solid media ...... 88 



Subcultures 91 



CHAPTER IX 

 THE MICROSCOPICAL EXAMINATION OF BACTERIA 



The history of the microscope 93 



The bacteriological microscope 93 



Oil immersion lens 93 



Abbe condenser 93 



The examination of living bacteria 94 



The principles of staining 98 



